UMR1287 EGFV, Bordeaux Sciences Agro, INRAE, University of Bordeaux, Villenave d'Ornon, France.
UMR1332 BFP, INRAE, University of Bordeaux, Villenave d'Ornon, France.
BMC Plant Biol. 2020 Jan 29;20(1):43. doi: 10.1186/s12870-020-2258-0.
Grapevine is a crop of major economic importance, yet little is known about the regulation of shoot development in grapevine or other perennial fruits crops. Here we combine genetic and genomic tools to identify candidate genes regulating shoot development in Vitis spp.
An F2 population from an interspecific cross between V. vinifera and V. riparia was phenotyped for shoot development traits, and three Quantitative Trait Loci (QTLs) were identified on linkage groups (LGs) 7, 14 and 18. Around 17% of the individuals exhibited a dwarfed phenotype. A transcriptomic study identified four candidate genes that were not expressed in dwarfed individuals and located within the confidence interval of the QTL on LG7. A deletion of 84,482 bp was identified in the genome of dwarfed plants, which included these four not expressed genes. One of these genes was VviCURLY LEAF (VviCLF), an orthologue of CLF, a regulator of shoot development in Arabidopsis thaliana.
The phenotype of the dwarfed grapevine plants was similar to that of clf mutants of A. thaliana and orthologues of the known targets of CLF in A. thaliana were differentially expressed in the dwarfed plants. This suggests that CLF, a major developmental regulator in A. thaliana, also controls shoot development in grapevine.
葡萄是一种具有重要经济意义的作物，但人们对葡萄或其他多年生水果作物的枝条发育调控知之甚少。在这里，我们结合遗传和基因组工具来鉴定调控葡萄属植物枝条发育的候选基因。
对 V. vinifera 和 V. riparia 种间杂交的 F2 群体进行了枝条发育性状的表型分析，在连锁群 7、14 和 18 上鉴定到了三个数量性状位点（QTL）。约 17%的个体表现出矮小的表型。转录组研究鉴定到四个候选基因，它们在矮小个体中不表达，并且位于 LG7 上 QTL 的置信区间内。在矮小植株的基因组中发现了一个 84482bp 的缺失，其中包含这四个不表达的基因。这些基因之一是 VviCURLY LEAF（VviCLF），它是拟南芥枝条发育调控因子 CLF 的同源基因。
矮小葡萄植株的表型与拟南芥 clf 突变体相似，并且在矮小植株中，CLF 在拟南芥中的已知靶基因的同源物的表达水平存在差异。这表明，拟南芥中主要的发育调控因子 CLF 也控制着葡萄的枝条发育。
