Zhao Y H, Su K, Guo Y H, Ma H F, Guo X W
Department of Pomology College of Horticulture, Shenyang Agricultural University, Shenyang, China.
Dalian Academy of Agricultural Science, Dalian, China.
Genet Mol Res. 2016 Jun 20;15(2):gmr8040. doi: 10.4238/gmr.15028040.
In this study, we aimed at finding the genetic regularity of grape maturation period. Early-maturing grapevine, "87-1", was used as the female parent and late-maturing, "9-22", as the male parent, to create an F1 hybrid population. A total of 149 individual plants and their parents were selected as the mapping population. Sequence-related amplified polymorphism and simple-sequence repeat analyses were performed. We performed a linkage analysis and constructed a molecular genetic map. In the obtained map, the female and male parents each covered 19 linkage groups containing 188 and 175 maker loci, respectively. The total map distances for the female and male parents were 1074.5 and 1100.2 cM, respectively, whereas the average genetic distances between each two loci were 5.7 and 7.8 cM, respectively. The interval-mapping method was used in a quantitative trait locus (QTL) analysis for fruit maturation period. A total of 12 QTLs associated with fruit maturation period were detected. These included four QTLs in the male parent genetic map that were located in linkage groups M5, M11, M14-1, and M16, with a 62.6-75.7% rate of contribution of each QTL. Another three QTLs were found in the female parent genetic map, located in linkage groups F6, F14-1, and F18, with a 72.7-77.7% rate of contribution of each QTL. Five more QTLs were detected in the consensus map, located in linkage groups LG11, LG14-1, LG16, LG17, and LG18, with 8.9-75.7% phenotypic variance explained by each QTL.
在本研究中，我们旨在探寻葡萄成熟期的遗传规律。以早熟葡萄品种“87 - 1”为母本，晚熟品种“9 - 22”为父本，构建了F1杂交群体。共选取149株单株及其亲本作为作图群体，进行了序列相关扩增多态性和简单序列重复分析。我们进行了连锁分析并构建了分子遗传图谱。在所得图谱中，母本和父本各自覆盖19个连锁群，分别包含188个和175个标记位点。母本和父本的总图距分别为1074.5 cM和1100.2 cM，而每两个位点之间的平均遗传距离分别为5.7 cM和7.8 cM。采用区间作图法对果实成熟期进行数量性状位点（QTL）分析，共检测到12个与果实成熟期相关的QTL。其中，在父本遗传图谱中有4个QTL，分别位于连锁群M5、M11、M14 - 1和M16上，每个QTL的贡献率为62.6% - 75.7%。在母本遗传图谱中发现另外3个QTL，位于连锁群F6、F14 - 1和F18上，每个QTL的贡献率为72.7% - 77.7%。在整合图谱中又检测到5个QTL，位于连锁群LG11、LG14 - 1、LG16、LG17和LG18上，每个QTL解释的表型变异为8.9% - 75.7%。
