Biofouling and Thermal Ecology Section, Water and Steam Chemistry Division, Bhabha Atomic Research Centre Facilities, Kalpakkam, India.
Life sciences Department, Homi Bhabha National Institute, Anushaktinagar, Mumbai, India.
Biofouling. 2020 Jan;36(1):56-72. doi: 10.1080/08927014.2020.1715371. Epub 2020 Jan 30.
The effects of two prominent copper oxide nanoparticles (CuO-NP and CuO-NP), with the oxidation state of Cu (cupric) and Cu (cuprous), on were evaluated. CuO-NP and CuO-NP were synthesized and characterized by XRD, FESEM, HR-TEM and Zeta potential. At sub-MIC (50 µg ml), both cupric and cuprous oxide NPs prevented yeast-to-hyphae switching and wrinkling behaviour in . The mechanism for the antifungal action of the two NPs differed; CuO-NP significantly elicited reactive oxygen species, whereas membrane damage was more pronounced with CuO-NP. Real time PCR analysis revealed that CuO-NP suppressed the morphological switching of yeast-to-hyphae by down-regulating , and and by up-regulation of the negative regulator . In comparison, CuO-NP resulted in down-regulation of and up-regulation of the negative regulators and . Between the two NPs, CuO exhibited increased antifungal activity due to its stable oxidation state (Cu) and its smaller dimensions compared with CuO-NP.
研究了两种具有代表性的氧化铜纳米粒子（CuO-NP 和 CuO-NP），其铜的氧化态分别为 Cu（二价铜）和 Cu（一价铜），对 的影响。通过 XRD、FESEM、HR-TEM 和 Zeta 电位对 CuO-NP 和 CuO-NP 进行了合成和表征。在亚 MIC（50μg/ml）浓度下，两种氧化铜纳米粒子均能阻止酵母向菌丝的转化和 的皱缩行为。这两种纳米粒子的抗真菌作用机制不同；CuO-NP 显著诱导线粒体产生活性氧，而 CuO-NP 则更明显地导致细胞膜损伤。实时 PCR 分析表明，CuO-NP 通过下调 和 以及上调负调控因子 来抑制酵母向菌丝的形态转变。相比之下，CuO-NP 导致 和负调控因子 和 的下调和上调。与 CuO-NP 相比，CuO 由于其稳定的氧化态（Cu）和较小的尺寸，表现出更高的抗真菌活性。
