Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
Food Microbiol. 2020 Jun;88:103411. doi: 10.1016/j.fm.2019.103411. Epub 2019 Dec 30.
Fungal pathogens lead to severe quality deterioration and yield loss, making it urgent to explore efficient measures to control fungal diseases at the preharvest and postharvest stages of plants. Therefore, studies on natural substances targeting alternative antimicrobial targets have become hot spots of research. Here, we show that honokiol, a polyphenolic compound obtained from Magnolia officinalis, significantly suppressed mycelial growth and reduced virulence of B. cinerea on harvested fruit by inducing autophagic activities and apoptosis. Moreover, honokiol was capable of abolishing the mitochondrial membrane potential and inducing the accumulation of reactive oxygen species. Some key genes involved in pathogenicity on fruit were also found significantly down-regulated. In summary, honokiol was effective as an alternative agent targeting autophagic and apoptotic machineries to control the incidence of gray mold, which may further enrich the toolkit of crop managers for fighting postharvest diseases caused by this and similar fungi.
真菌病原体导致严重的质量恶化和产量损失，因此迫切需要探索有效的措施来控制植物采前和采后阶段的真菌病害。因此，针对替代抗菌靶点的天然物质的研究已成为研究热点。在这里，我们表明，从厚朴中获得的多酚化合物厚朴酚通过诱导自噬活性和细胞凋亡，显著抑制了菌核菌的菌丝生长并降低了其对收获果实的毒力。此外，厚朴酚能够破坏线粒体膜电位并诱导活性氧的积累。还发现一些与果实致病性相关的关键基因显著下调。总之，厚朴酚是一种有效的替代剂，可靶向自噬和细胞凋亡机制来控制灰霉病的发生，这可能进一步丰富作物管理者防治由这种和类似真菌引起的采后病害的工具包。
