Bae In-Ho, Jeong Myung Ho, Park Dae Sung, Lim Kyung Seob, Shim Jae Won, Kim Mun Ki, Park Jun-Kyu
1Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare, Jaebongro 671, Dong-gu, Gwangju, 61469 Republic of Korea.
Korea Cardiovascular Stent Research Institute, Jangsung, 57248 Republic of Korea.
Biomater Res. 2020 Jan 23;24:4. doi: 10.1186/s40824-020-0182-x. eCollection 2020.
The aim of this study was to characterize the mechanical and physio-biological properties of peptide-coated stent (PCS) compared to commercialized drug-eluting stents (DESs).
WKYMVm (Trp-Lys-Tyr-Met-Val-D-Met), a stimulating peptide for homing endothelial colony-forming cell was specially synthesized and coated to bare metal stent (BMS) by dopamine-derived coordinated bond. Biological effects of PCS were investigated by endothelial cell proliferation assay and pre-clinical animal study. And mechanical properties were examined by various experiment.
The peptide was well-coated to BMS and was maintained and delivered to 21 and 7 days in vitro and in vivo, respectively. Moreover, the proliferation of endothelial cell in PCS group was increased (approximately 36.4 ± 5.77%) in PCS group at 7 day of culture compare to BMS. Although, the radial force of PCS was moderated among study group. The flexibility of PCS was (0.49 ± 0.082 N) was greatest among study group. PCS did not show the outstanding performance in recoil and foreshortening test (3.1 ± 0.22% and 2.1 ± 0.06%, respectively), which was the reasonable result under the guide line of FDA (less than 7.0%). The nominal pressure (3.0 mm in a diameter) of PCS established by compliance analysis was 9 atm. The changing of PCS diameter by expansion was similar to other DESs, which is less than 10 atm of pressure for the nominal pressure.
These results suggest that the PCS is not inferior to commercialized DES. In addition, since the PCS was fabricated as polymer-free process, secondary coating with polymer-based immunosuppressive drugs such as -limus derivatives may possible.
本研究旨在表征与商业化药物洗脱支架（DES）相比，肽涂层支架（PCS）的机械性能和生理生物学特性。
专门合成了一种用于归巢内皮祖细胞的刺激肽WKYMVm（色氨酸-赖氨酸-酪氨酸-甲硫氨酸-缬氨酸-D-甲硫氨酸），并通过多巴胺衍生的配位键将其涂覆在裸金属支架（BMS）上。通过内皮细胞增殖试验和临床前动物研究来研究PCS的生物学效应。并通过各种实验检测其机械性能。
该肽很好地涂覆在BMS上，分别在体外和体内维持并递送21天和7天。此外，与BMS相比，培养7天时PCS组内皮细胞的增殖增加（约36.4±5.77%）。尽管如此，PCS的径向力在研究组中适中。PCS的柔韧性（0.49±0.082 N）在研究组中最大。PCS在回缩和缩短试验中未表现出突出性能（分别为3.1±0.22%和2.1±0.06%），这是在FDA指导原则（小于7.0%）下的合理结果。通过顺应性分析确定的PCS标称压力（直径3.0 mm）为9个大气压。PCS直径随扩张的变化与其他DES相似，对于标称压力，压力变化小于10个大气压。
这些结果表明PCS并不逊色于商业化DES。此外，由于PCS是通过无聚合物工艺制造的，因此可能可以用基于聚合物的免疫抑制药物（如雷帕霉素衍生物）进行二次涂层。
