[具体物质]对HL-60/MX2细胞凋亡诱导及多药耐药逆转的影响
The Effects of on the Apoptosis Induction and the Reversal of Multidrug Resistance in HL-60/MX2.
作者信息
Hyun Jae-Hee, Kang Jung-Il, Kim Sang-Cheol, Kim Elvira, Kang Ji-Hoon, Kwon Jung-Mi, Park Doek-Bae, Lee Young-Jae, Yoo Eun-Sook, Kang Hee-Kyoung
机构信息
15Department of Medicine, College of Medicine, Cheju National University, 66 Jejudaehakno, Jeju, 690-756 Korea.
25Department of Veterinary Medicine, College of Applied Life Science, Cheju National University, Jeju, 690-756 Korea.
出版信息
Toxicol Res. 2008 Mar;24(1):29-36. doi: 10.5487/TR.2008.24.1.029. Epub 2008 Mar 1.
The present study investigated the anti-proliferative and chemosensitizing effects of var. against multi-drug resistant (MDR) cancer cells. The 80% methanol extract, chloroform (CHCI) fraction and butanol (BuOH) fraction of inhibited the growth of mitoxantrone (MX) resistant HL-60 (HL-60/MX2) cells. When HL-60/MX2 cells were treated with the CHCI and BuOH fractions, DNA ladder and sub-G1 hypodiploid cells were observed. Furthermore, the fractions reduced Bcl-2 mRNA levels, whereas Bax mRNA levels were increased. These results suggest that the inhibitory effect of on the growth of the HL-60/MX2 cells might arise from the induction of apoptosis. Treatment of HL-60/MX2 cells with the fractions markedly decreased the mRNA levels of the multi-drug resistance protein-1 and breast cancer resistance protein. The CHCI3 fraction and hexane fraction increased MX accumulation in HL-60/MX2 cells. These results imply that the CHCI fraction of plays a pivotal role as a chemosensitizer. We suggest that components of might have a therapeutic potential for the treatment of MDR leukemia.
本研究调查了[植物名称]变种对多药耐药（MDR）癌细胞的抗增殖和化学增敏作用。[植物名称]的80%甲醇提取物、氯仿（CHCI）组分和丁醇（BuOH）组分抑制了米托蒽醌（MX）耐药的HL-60（HL-60/MX2）细胞的生长。当用CHCI和BuOH组分处理HL-60/MX2细胞时，观察到DNA梯带和亚G1期亚二倍体细胞。此外，这些组分降低了Bcl-2 mRNA水平，而Bax mRNA水平升高。这些结果表明，[植物名称]对HL-60/MX2细胞生长的抑制作用可能源于细胞凋亡的诱导。用这些组分处理HL-60/MX2细胞显著降低了多药耐药蛋白-1和乳腺癌耐药蛋白的mRNA水平。CHCI3组分和己烷组分增加了HL-60/MX2细胞中MX的积累。这些结果表明，[植物名称]的CHCI组分作为化学增敏剂起着关键作用。我们认为，[植物名称]的成分可能对多药耐药白血病的治疗具有治疗潜力。
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