Rodríguez Estévez Marta, Gummerlich Nils, Myronovskyi Maksym, Zapp Josef, Luzhetskyy Andriy
Pharmaceutical Biotechnology, University of Saarland, Saarbrücken, Germany.
Department of Pharmacy, Institute of Pharmaceutical Biology, University of Saarland, Saarbrücken, Germany.
Front Chem. 2020 Jan 10;7:896. doi: 10.3389/fchem.2019.00896. eCollection 2019.
Streptomycetes constitute a diverse bacterial group able to produce a wide variety of secondary metabolites with potential applications in the pharmacy industry. However, the genes responsible for the biosynthesis of these compounds are very frequently inactive or expressed at very low levels under standard laboratory cultivation conditions. Therefore, the activation or upregulation of secondary metabolite biosynthesis genes is a crucial step for the discovery of new bioactive natural products. We have recently reported the discovery of the biosynthetic genes for the antibiotic nybomycin ( genes) in subsp. . The genes were expressed in the heterologous host Del14, which produces not only nybomycin, but also a novel compound. In this study, we describe the isolation, purification, and structure elucidation of the new substance named benzanthric acid.
链霉菌是一类多样化的细菌群体，能够产生多种具有潜在制药工业应用价值的次级代谢产物。然而，在标准实验室培养条件下，负责这些化合物生物合成的基因常常处于无活性状态或表达水平极低。因此，激活或上调次级代谢产物生物合成基因是发现新的生物活性天然产物的关键步骤。我们最近报道了在亚种中发现抗生素尼博霉素（基因）的生物合成基因。这些基因在异源宿主Del14中表达，Del14不仅产生尼博霉素，还产生一种新型化合物。在本研究中，我们描述了名为苯并蒽酸的新物质的分离、纯化和结构解析。
