Center of Medicine Clinical Research, Department of Pharmacy, PLA General Hospital, Beijing, China.
Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China.
Biomed Res Int. 2020 Jan 3;2020:5916260. doi: 10.1155/2020/5916260. eCollection 2020.
biofilms on inserted devices have been proposed as one of the important factors for hospital-acquired infections, which cause increased resistance to currently used antibiotics. Therefore, it is urgently necessary to develop new treatments with more efficient bacterial clearance. In the present study, we aimed at investigating whether low-frequency ultrasound (LFU) could enhance the bactericidal activity of antimicrobial agents (meropenem (MEM), tigecycline (TGC), fosfomycin (FOM), amikacin (AMK), and colistin (COL)) against biofilm infection. biofilm was cultivated on the catheter . Synergistic effects were observed in groups of single ultrasound (S-LFU, 5 min) or multiple ultrasound (M-LFU, 5 min every 8 h (q8h)) in combination with MEM, TGC, and FOM. However, AMK and COL did not show the synergistic effect with either S-LFU or M-LFU. S-LFU in combination with FOM only significantly decreased bacterial counts right after ultrasound, while M-LFU could prolong the synergistic effect until 24 h. The results showed that LFU in combination with antimicrobial agents had a synergistic effect on biofilm, and M-LFU might extend the time of synergistic effect compared with S-LFU.
植入设备上的生物膜被认为是医院获得性感染的重要因素之一，这导致了目前使用的抗生素的耐药性增加。因此，迫切需要开发具有更高细菌清除效率的新治疗方法。在本研究中，我们旨在研究低频超声（LFU）是否可以增强抗菌药物（美罗培南（MEM）、替加环素（TGC）、磷霉素（FOM）、阿米卡星（AMK）和多粘菌素（COL））对生物膜感染的杀菌活性。在导管上培养生物膜。单独超声（S-LFU，5 分钟）或多次超声（M-LFU，每 8 小时（q8h）超声 5 分钟）联合 MEM、TGC 和 FOM 时观察到协同作用。然而，AMK 和 COL 与 S-LFU 或 M-LFU 均未显示协同作用。S-LFU 联合 FOM 仅在超声后立即显著降低细菌计数，而 M-LFU 可延长协同作用至 24 小时。结果表明，LFU 联合抗菌药物对生物膜具有协同作用，与 S-LFU 相比，M-LFU 可能延长协同作用时间。
