Ultrashort Laser Nano Manufacturing Research Facility, Department of Mechanical and Industrial Engineering, Ryerson University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada; Nano Characterization Laboratory, Department of Aerospace Engineering, Ryerson University, 350 Victoria Street, Toronto, Ontario, M5B 2K3, Canada; Nano Bio Interface Facility, Department of Mechanical and Industrial Engineering, Ryerson University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada.
Ultrashort Laser Nano Manufacturing Research Facility, Department of Mechanical and Industrial Engineering, Ryerson University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada; Keenan Research Center, St. Michael's Hospital, 209 Victoria Street, Toronto, Ontario, M5B 1T8, Canada.
Biosens Bioelectron. 2020 Mar 1;151:111967. doi: 10.1016/j.bios.2019.111967. Epub 2019 Dec 14.
This article presents a unique 3D biocompatible Aluminum-based quantum structure (QS) for in vitro cancer detection using Surface Enhanced Raman Scattering (SERS). The Al-based QSs fabricated using ultrashort pulsed laser are of two distinct surface characters, wrinkled and smooth spherical. The limit of detection for chemical sensing of Crystal Violet and Rhodamine 6G by the Al-QS was driven up to single molecule sensing (femtomolar concentration). Biological sensing of cysteine, a disease biomarker and carcinoembryonic antigen (CEA), a cancer biomarker was also tested by the Al-QS. The ability of in vitro cell detection using Al-QS was analyzed with three cell lines, mammalian fibroblast and pancreatic and lung cancer cells. The Al-QS were up taken by the cells through label-free self-internalization and were sensed by SERS. Further assay was performed to differentiate cancerous and non-cancerous cells by measuring lipid and protein peak intensity within the cells. The result of this research indicated that SERS based Al-QS could be a suitable candidate for the early diagnosis of cancer.
本文提出了一种独特的 3D 生物相容性基于铝的量子结构（QS），用于使用表面增强拉曼散射（SERS）进行体外癌症检测。使用超短脉冲激光制造的基于铝的 QS 具有两种不同的表面特性，即皱纹和光滑的球形。铝 QS 对 Crystal Violet 和 Rhodamine 6G 的化学传感的检测限提高到单分子传感（飞摩尔浓度）。还通过 Al-QS 测试了生物传感中的半胱氨酸，一种疾病生物标志物和癌胚抗原（CEA），一种癌症生物标志物。使用三种细胞系（哺乳动物成纤维细胞和胰腺癌细胞和肺癌细胞）分析了使用 Al-QS 进行体外细胞检测的能力。Al-QS 通过无标记的自内化被细胞摄取，并通过 SERS 进行感测。通过测量细胞内脂质和蛋白质峰强度来进行进一步的分析，以区分癌性和非癌性细胞。这项研究的结果表明，基于 SERS 的 Al-QS 可能是癌症早期诊断的合适候选者。
