Sato Aya, Kawata Masakado
Faculty of Education, Gunma University, 4-2 Aramaki, Maebashi, Gunma, 371-8510, Japan.
Graduate School of Life Sciences, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.
BMC Res Notes. 2020 Jan 30;13(1):49. doi: 10.1186/s13104-020-4909-5.
The purpose of this study was to segregate the genetic lines responsible for the orange area of coloration in males and the response to orange coloration exhibited by females in the guppy (Poecilia reticulata) through artificial selection. This study is part of a project that uses QTL-seq to search for candidate genes involved in male orange coloration and female response to male coloration. We created two lines: high-selected lines of males having large areas of orange spots and of females with high response to male orange coloration; and low-selected lines of males having small areas of orange spots and of females with low response to male orange coloration.
The male orange area and the female response became significantly different between high- and low-selected lines after three generations of artificial selection. This indicates that the differences in the frequencies of alleles at loci affecting the orange area and the female response between the lines increased over the generations through selection.
本研究的目的是通过人工选择，分离出孔雀鱼（孔雀花鳉）中导致雄性橙色区域着色的遗传品系以及雌性对橙色着色的反应。本研究是一个项目的一部分，该项目使用QTL-seq来寻找参与雄性橙色着色和雌性对雄性着色反应的候选基因。我们创建了两个品系：具有大面积橙色斑点的雄性高选择品系和对雄性橙色着色反应高的雌性高选择品系；以及具有小面积橙色斑点的雄性低选择品系和对雄性橙色着色反应低的雌性低选择品系。
经过三代人工选择后，高选择品系和低选择品系之间的雄性橙色区域和雌性反应变得显著不同。这表明，通过选择，影响橙色区域的基因座上等位基因频率的差异以及品系之间雌性反应的差异在几代中增加。
