Rabiei Mitra, Zarrini Gholamreza, Mahdavi Majid
Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.
Adv Pharm Bull. 2020 Jan;10(1):125-129. doi: 10.15171/apb.2020.016. Epub 2019 Dec 11.
The present study was mainly designed to assess anti-cancer effects of lactobacilli isolated from traditional dairy products, on HCT116 colorectal cancer cell lines. Traditional dairy products samples were collected from the region of Azarbayjan and the suspensions were cultured in MRS agar medium. The isolates were identified by biochemical and molecular methods. Isolated bacteria were cultured in MRS broth. Supernatants of the isolates cultures were collected and their cytotoxicity was evaluated on HCT116 cancer cells. Morphological changes of the treated cells by supernatant were observed using an inverted microscope. Cell metabolic activity was assessed by MTT assay. The morphology of apoptotic cells was examined using a fluorescent microscope. In cell cycle analysis, content measurement of DNA was performed by flow cytometry. Out of 30 lactobacilli were isolated from dairy products samples, six isolates belong to curd samples. Cell-based assays showed that culture supernatant of one isolate (UT1) had a significant anticancer effect on colorectal HCT116 cell lines (<0.05). The 16S rRNA sequence analysis revealed that the isolate UT1 was 99% compatible with Lactobacillus casei. It is noteworthy that the supernatant of L. casei UT1 can be candidate for studies on compounds having anti-cancer effect.
本研究主要旨在评估从传统乳制品中分离出的乳酸杆菌对HCT116结肠癌细胞系的抗癌作用。从阿塞拜疆地区收集传统乳制品样本，并将悬浮液在MRS琼脂培养基中培养。通过生化和分子方法对分离株进行鉴定。将分离出的细菌在MRS肉汤中培养。收集分离株培养物的上清液，并评估其对HCT116癌细胞的细胞毒性。使用倒置显微镜观察经上清液处理的细胞的形态变化。通过MTT法评估细胞代谢活性。使用荧光显微镜检查凋亡细胞的形态。在细胞周期分析中，通过流式细胞术进行DNA含量测量。从乳制品样本中分离出的30株乳酸杆菌中，有6株分离株属于凝乳样本。基于细胞的试验表明，一种分离株（UT1）的培养上清液对结肠HCT116细胞系具有显著的抗癌作用（<0.05）。16S rRNA序列分析显示，分离株UT1与干酪乳杆菌的兼容性为99%。值得注意的是，干酪乳杆菌UT1的上清液可作为具有抗癌作用的化合物研究的候选物。
