从发酵芥菜中分离出的乳酸菌对降低胆固醇的作用。
Effects of lactic acid bacteria isolated from fermented mustard on lowering cholesterol.
作者信息
Wang Shu Chen, Chang Chen Kai, Chan Shu Chang, Shieh Jiunn Shiuh, Chiu Chih Kwang, Duh Pin-Der
机构信息
Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, Taiwan.
Department of Biotechnology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, Taiwan.
出版信息
Asian Pac J Trop Biomed. 2014 Jul;4(7):523-8. doi: 10.12980/APJTB.4.201414B54.
OBJECTIVE
To evaluate the ability of lactic acid bacteria (LAB) strains isolated from fermented mustard to lower the cholesterol in vitro.
METHODS
The ability of 50 LAB strains isolated from fermented mustard on lowering cholesterol in vitro was determined by modified o-phtshalaldehyde method. The LAB isolates were analyzed for their resistance to acid and bile salt. Strains with lowering cholesterol activity, were determined adherence to Caco-2 cells.
RESULTS
Strain B0007, B0006 and B0022 assimilated more cholesterol than BCRC10474 and BCRC 17010. The isolated strains showed tolerance to pH 3.0 for 3 h despite variations in the degree of viability and bile-tolerant strains, with more than 10(8) CFU/mL after incubation for 24 h at 1% oxigall in MRS. In addition, strain B0007 and B0022 identified as Lactobacillus plantarum with 16S rDNA sequences were able to adhere to the Caco-2 cell lines.
CONCLUSIONS
These strains B0007 and B0022 may be potential functional sources for cholesterol-lowering activities as well as adhering to Caco-2 cell lines.
目的
评估从发酵芥菜中分离出的乳酸菌菌株在体外降低胆固醇的能力。
方法
采用改良邻苯二甲醛法测定从发酵芥菜中分离出的50株乳酸菌菌株在体外降低胆固醇的能力。分析乳酸菌分离株对酸和胆盐的抗性。对具有降低胆固醇活性的菌株，测定其对Caco-2细胞的黏附性。
结果
菌株B0007、B0006和B0022比BCRC10474和BCRC 17010吸收更多的胆固醇。尽管存活程度存在差异，但分离出的菌株在pH 3.0条件下耐受3小时，且具有胆盐耐受性，在含1%氧胆酸盐的MRS培养基中于24小时培养后，活菌数超过10⁸CFU/mL。此外，经16S rDNA序列鉴定为植物乳杆菌的菌株B0007和B0022能够黏附于Caco-2细胞系。
结论
这些菌株B0007和B0022可能是具有降低胆固醇活性以及黏附于Caco-2细胞系的潜在功能来源。
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