利用 CRISPR/Cas9 技术生成基因编辑小鼠。

Generation of Genetically Modified Mice Using CRISPR/Cas9.

机构信息

National Centre for Biotechnology (CNB-CSIC) and Biomedical Research Networking Centre for Rare Diseases (CIBERER-ISCIII), Madrid, Spain.

出版信息

Methods Mol Biol. 2020;2110:129-138. doi: 10.1007/978-1-0716-0255-3_9.

DOI:10.1007/978-1-0716-0255-3_9

PMID:32002906

Abstract

Using CRISPR-based genome-editing techniques, we are able to generate a variety of new mouse models of several types of diseases. These animal models will be instrumental not only for enabling the comprehension of a particular disease and its underlying molecular mechanism but also as unique recipients for testing novel and innovative therapeutic approaches that are being currently explored. This chapter describes detailed step-by-step protocols, reagents, and equipment required for successful generation of genome-edited mice using CRISPR tools.

摘要

利用基于 CRISPR 的基因组编辑技术，我们能够生成多种不同类型疾病的新型小鼠模型。这些动物模型不仅有助于理解特定疾病及其潜在的分子机制，而且还可以作为正在探索的新型创新治疗方法的独特受体。本章详细描述了使用 CRISPR 工具成功生成基因组编辑小鼠所需的详细步骤、试剂和设备。