Katigbak Alexandra, Robert Francis, Paquet Marilène, Pelletier Jerry
Department of Biochemistry, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.
Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.
G3 (Bethesda). 2018 May 4;8(5):1627-1635. doi: 10.1534/g3.117.300327.
Genetically engineered mouse models (GEMMs) are powerful tools by which to probe gene function , obtain insight into disease etiology, and identify modifiers of drug response. Increased sophistication of GEMMs has led to the design of tissue-specific and inducible models in which genes of interest are expressed or ablated in defined tissues or cellular subtypes. Here we describe the generation of a transgenic mouse harboring a doxycycline-regulated Cas9 allele for inducible genome engineering. This model provides a flexible platform for genome engineering since editing is achieved by exogenous delivery of sgRNAs and should allow for the modeling of a range of biological and pathological processes.
基因工程小鼠模型（GEMMs）是探究基因功能、深入了解疾病病因以及识别药物反应修饰因子的强大工具。GEMMs技术的日益成熟促使了组织特异性和诱导性模型的设计，在这些模型中，感兴趣的基因可在特定组织或细胞亚型中表达或缺失。在此，我们描述了一种携带强力霉素调控的Cas9等位基因的转基因小鼠的产生，用于诱导性基因组工程。该模型为基因组工程提供了一个灵活的平台，因为编辑是通过外源递送sgRNA实现的，并且应该能够对一系列生物学和病理过程进行建模。
