浓缩糖蜜发酵液和乳酸菌在玉米青贮生产中的应用。
Application of condensed molasses fermentation solubles and lactic acid bacteria in corn silage production.
机构信息
Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan.
Graduate Institute of Biotechnology, Chinese Culture University, Taipei, Taiwan.
出版信息
J Sci Food Agric. 2020 Apr;100(6):2722-2731. doi: 10.1002/jsfa.10304. Epub 2020 Feb 17.
BACKGROUND
The aim of this study is to investigate the application of two lactic acid bacteria and dry condensed molasses fermentation solubles (CMS) in the making and preservation of corn silage. Forage corn materials are divided into eight treatment groups as follows: (i) control, (ii) B2 (Lactobacillus plantarum B2, 1 × 10 cfu kg ), (iii) LAS (Lactobacillus buchneri 40788, 3 × 10 cfu kg ), (iv) B2 + LAS, (v) CMS (35 g kg , fresh weight), (vi) B2 + CMS, (vii) LAS + CMS and (viii) B2 + LAS + CMS. The silage composition and aerobic stability are determined after ensiling for 90 days. Furthermore, the digestibility of the silage product and gas production are evaluated using a trotro digestion procedure.
RESULTS
The assay results indicate that the CMS supplementation and B2 inoculation significantly increased lactic acid concentration (P < 0.01). However, they also reduced the content of acetic acid and silage pH at the initial fermentation stage. The CMS supplemented with B2 (B2 + CMS) showed an improvement in the quality of silage, but a significant decrease in aerobic stability (P < 0.01). The B2 + LAS + CMS treatment yielded an increase in acetic acid production during the late fermentation period and is able to extend the aerobic stability of corn silage. Furthermore, this study shows that CMS supplementation alone can significantly improve the digestibility of the in vitro dry matter (P < 0.01) and the microbial protein synthesis efficiency (P = 0.01). In addition, the CMS supplementation is beneficial for enhancing the aerobic stability of corn silage.
CONCLUSIONS
These results suggest that the combination of CMS supplementation and a suitable inoculation lactic acid bacterial strain can be highly promising for enhancing the higher quality and stability of corn silage. © 2020 Society of Chemical Industry.
背景
本研究旨在探讨两种乳酸菌和干缩糖蜜发酵液（CMS）在玉米青贮制作和保存中的应用。青贮饲料玉米材料分为以下 8 个处理组：（i）对照组，（ii）B2（植物乳杆菌 B2，1×10 cfu kg -1 ），（iii）LAS（布氏乳杆菌 40788，3×10 cfu kg -1 ），（iv）B2+LAS，（v）CMS（35 g kg -1 ，鲜重），（vi）B2+CMS，（vii）LAS+CMS 和（viii）B2+LAS+CMS。青贮 90 天后，测定青贮饲料的组成和有氧稳定性。此外，采用 trotro 消化程序评估青贮产品的消化率和产气量。
结果
结果表明，CMS 补充和 B2 接种显著增加了乳酸浓度（P<0.01）。然而，它们也降低了初始发酵阶段的乙酸含量和青贮饲料 pH 值。补充 B2 的 CMS（B2+CMS）表现出青贮饲料质量的改善，但有氧稳定性显著降低（P<0.01）。B2+LAS+CMS 处理在发酵后期产生了更多的乙酸，并且能够延长玉米青贮饲料的有氧稳定性。此外，本研究表明，CMS 单独添加可以显著提高体外干物质的消化率（P<0.01）和微生物蛋白合成效率（P=0.01）。此外，CMS 补充有利于提高玉米青贮饲料的有氧稳定性。
结论
这些结果表明，CMS 补充和适当接种乳酸菌菌株的组合对于提高玉米青贮饲料的质量和稳定性具有很高的应用前景。© 2020 英国化学工程师学会。
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