Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Pathog Glob Health. 2020 Feb;114(1):46-54. doi: 10.1080/20477724.2020.1719325. Epub 2020 Jan 31.
() is one of the main causative agents of pneumococcal diseases. To date, more than 90 distinct serotypes have been identified. Implementation of vaccines has caused a drastic reduction in vaccine-serotype pneumococcal diseases but increase in cases due to non-vaccine serotype has been observed in Malaysia. However, further investigation on different serotype incidence in Malaysia is needed and the rate of pneumococcal vaccination for new-born babies in Malaysia remains low. The recent emergence of drug-resistant (DRSP) has also been a global concern, especially penicillin resistance. This study determined the serotypes of strains ( = 95) isolated from nasopharyngeal specimens from children admitted to UMMC from 2013 to 2015. In accordance with previous studies, PCR result showed 40% of NT isolates were successfully typed as 3 less common serotypes, namely 9N/L, 17A, and 23B. The repetitive-element PCR (REP-PCR) result revealed genetic variations among the strains whereby five major clusters were observed at the similarity of 80% by clustering analysis based on fingerprint data. Penicillin-binding proteins (s) of selected isolates were studied by PCR and sequencing. Three strains with ≤19-mm diameter zone for Oxacillin Disc Diffusion (ODD) test previously were recorded to have mutation on all and with MIC of 4 µg/ml, which were penicillin-intermediate resistance according to the CLSI breakpoints.
肺炎链球菌是导致肺炎球菌疾病的主要病原体之一。迄今为止，已经鉴定出超过 90 种不同的血清型。疫苗的实施导致疫苗血清型肺炎球菌疾病的发病率大幅下降，但在马来西亚，观察到非疫苗血清型的病例有所增加。然而，需要进一步调查马来西亚不同血清型的发病率，马来西亚新生儿的肺炎球菌疫苗接种率仍然很低。最近，耐药性肺炎链球菌（DRSP）的出现也引起了全球关注，尤其是青霉素耐药性。本研究从 2013 年至 2015 年期间，从 UMMC 收治的儿童鼻咽标本中分离出的 株 （=95 株）进行了血清型分析。与以往的研究一致，PCR 结果显示，40%的 NT 分离株成功地分为 3 种较少见的血清型，即 9N/L、17A 和 23B。重复元件 PCR（REP-PCR）结果显示，菌株之间存在遗传变异，聚类分析显示基于指纹数据的相似度为 80%时，观察到 5 个主要聚类。对部分分离株的青霉素结合蛋白（s）进行了 PCR 和测序研究。此前，有 3 株 ODD 试验的抑菌环直径（ODD）≤19mm，记录到所有 和 上均有突变，MIC 为 4µg/ml，根据 CLSI 折点，这是青霉素中介耐药。
