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利用光学显微镜和扫描电子显微镜对喜马拉雅山西部德奥塞高原高山带一些唇形科植物的花粉和叶片微形态进行比较研究。

Comparative pollen and foliar micromorphological studies using light microscopy and scanning electron microscopy of some selected species of Lamiaceae from Alpine Zone of Deosai Plateau, Western Himalayas.

机构信息

Department of Botany, FG Degree College for Women, Skardu, Pakistan.

Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

出版信息

Microsc Res Tech. 2020 Jun;83(6):579-588. doi: 10.1002/jemt.23448. Epub 2020 Jan 31.

DOI:10.1002/jemt.23448
PMID:32003533
Abstract

The study was conducted to highlight a detailed account of morphology of pollen chosen species of Lamiaceae through scanning electron microscopy, and the anatomical characteristics of leaf epidermis of seven species using simple light microscopy. In results, Anisomeles indica and Otostegia aucheri belong to subfamily Lamioideae because it has tricolpate pollen while the rest eight species belong to subfamily Nepetoideae (hexacolpate pollen). The exine sculpturing of pollen of studied species was found to be reticulate. In the family Lamiaceae, four kinds of stomata were found anomocytic, anisocytic, diacytic, and actinocytic, respectively. The cell wall patterns of epidermal cells were irregular or polygonal with straight or undulate walls. It was noted that the variety of the epidermal trichomes seems of taxonomically important for the identification of species of Lamiaceae. Both nonglandular and glandular trichomes were analyzed. The nonglandular trichomes were characterized with long, thin, and pointed apical unicellular cells. The nonglandular trichomes were A-shaped in Thymus linearis. In Perovskia abrotanoides, stellate glandular trichomes were observed whereas in A. indica and Mentha royleana both glandular and nonglandular trichomes were found. In A. indica, the nonglandular trichomes were sessile and peltate in M. royleana. For the first time in this study, pollen and foliar micromorphological features of selected species of this area are carried out. These taxonomic characters were found to be important in discrimination of species from each other. In future, the detailed study with comprehensive morphology coupled with other important characters is required for delimitation of taxa at various levels.

摘要

该研究旨在通过扫描电子显微镜详细描述所选唇形科植物花粉的形态,以及使用简单的光学显微镜观察七种植物叶片表皮的解剖特征。结果表明,Anisomeles indica 和 Otostegia aucheri 属于 Lamioideae 亚科,因为它们具有三沟花粉,而其余八种属于 Nepetoideae 亚科(六沟花粉)。研究中发现,花粉的外壁纹饰为网状。在唇形科中,发现了四种类型的气孔,分别为无规则型、不等型、双环型和辐射型。表皮细胞的细胞壁模式为不规则或多边形,具有直壁或波状壁。值得注意的是,表皮毛的多样性对于唇形科物种的鉴定似乎具有分类学上的重要性。对非腺毛和腺毛进行了分析。非腺毛具有长而细、顶端单细胞的特点。Thymus linearis 中的非腺毛呈 A 形。在 Perovskia abrotanoides 中观察到星状腺毛,而在 A. indica 和 Mentha royleana 中则发现了腺毛和非腺毛。在 A. indica 中,非腺毛无柄且具盾形。本研究首次对该地区所选物种的花粉和叶片微形态特征进行了研究。这些分类特征对于区分物种具有重要意义。未来,需要结合其他重要特征进行详细的综合形态学研究,以在各个水平上对分类群进行界定。

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