Department of Environmental Science and Engineering, School of Space and Environment, Beihang University, Beijing, 10191, China; Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Beihang University, Beijing, 100191, China.
Department of Environmental Science and Engineering, School of Space and Environment, Beihang University, Beijing, 10191, China.
J Hazard Mater. 2020 May 5;389:122155. doi: 10.1016/j.jhazmat.2020.122155. Epub 2020 Jan 22.
As a novel antioxidant, hydrogen water has been widely used to alleviate oxidative stress in plants as well as in the medical field. However, the function of hydrogen water in environmental toxicology remains unknown. In this study, combining nanobubbles (NBs) and hydrogen water, we investigate the effect and mechanism of hydrogen NB water on copper induced acute toxicity to water fleas (Daphnia magna). The 24-h lethal Cu concentrations at which 50 % of the population die were 84 μg/L in hydrogen NB water and 45 μg/L in control water, confirming that hydrogen NB water effectively alleviated acute Cu toxicity in D. magna. The results were consistent with a significant reduction of Cu uptake and decrease of Cu accumulation in D. magna. As confirmed in fluorescence spectrophotometry and high-content screening system analysis, the hydrogen NB water also significantly reduced the oxidative damage and improved Cu tolerance in D. magna. From the results, it can be inferred that hydrogen NB water alleviates Cu stress in D. magna by depressing Cu bioaccumulation and reducing oxidative stress. The results provide basic data of hydrogen NB water for environmental toxicologists, and also a reference for the application of hydrogen NB water in the environment.
作为一种新型抗氧化剂，氢气水已被广泛用于缓解植物和医学领域的氧化应激。然而，氢气水在环境毒理学中的作用尚不清楚。在这项研究中，我们结合纳米气泡（NBs）和氢气水，研究了氢气 NB 水对铜诱导的水蚤（大型溞）急性毒性的作用和机制。在氢气 NB 水中，24 小时致死 Cu 浓度为 50%的种群死亡的浓度为 84 μg/L，而在对照水中为 45 μg/L，这证实了氢气 NB 水有效地缓解了 D. magna 中的急性 Cu 毒性。结果与 D. magna 中 Cu 摄取量的显著减少和 Cu 积累量的降低一致。荧光分光光度法和高通量筛选系统分析也证实，氢气 NB 水还显著降低了 D. magna 的氧化损伤，提高了其对 Cu 的耐受性。从结果可以推断，氢气 NB 水通过抑制 Cu 生物积累和降低氧化应激来缓解 D. magna 中的 Cu 胁迫。研究结果为环境毒理学家提供了氢气 NB 水的基础数据，也为氢气 NB 水在环境中的应用提供了参考。
