Cheng Pengfei, Wang Jun, Zhao Zhushan, Kong Lingshuai, Lou Wang, Zhang Tong, Jing Dedao, Yu Julong, Shu Zhaolin, Huang Liqin, Zhu Wenjiao, Yang Qing, Shen Wenbiao
Laboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Zhenjiang Institute of Agricultural Science of the Ning-Zhen Hilly District, Jurong 212400, China.
Plants (Basel). 2021 Oct 28;10(11):2331. doi: 10.3390/plants10112331.
How to use environmentally friendly technology to enhance rice field and grain quality is a challenge for the scientific community. Here, we showed that the application of molecular hydrogen in the form of hydrogen nanobubble water could increase the length, width, and thickness of brown/rough rice and white rice, as well as 1000-grain weight, compared to the irrigation with ditch water. The above results were well matched with the transcriptional profiles of representative genes related to high yield, including up-regulation of () for cellular proliferation, () for grain width, () for grain length and width, () for grain width and weight, and down-regulation of negatively correlated gene () for grain length. Meanwhile, although total starch content in white rice is not altered by HNW, the content of amylose was decreased by 31.6%, which was parallel to the changes in the transcripts of the amylose metabolism genes. In particular, cadmium accumulation in white rice was significantly reduced, reaching 52% of the control group. This phenomenon was correlated well with the differential expression of transporter genes responsible for Cd entering plants, including down-regulated (), ( and ), and (), and for decreasing Cd accumulation in grain, including down-regulated (). This study clearly showed that the application of molecular hydrogen might be used as an effective approach to increase field and grain quality of rice.
如何利用环保技术提高稻田和稻谷品质是科学界面临的一项挑战。在此，我们表明，与用沟渠水灌溉相比，以氢纳米气泡水形式施用分子氢可增加糙米/粗米和白米的长度、宽度和厚度，以及千粒重。上述结果与高产相关代表性基因的转录谱很好地匹配，包括促进细胞增殖的（）上调、控制粒宽的（）上调、控制粒长和粒宽的（）上调、控制粒宽和粒重的（）上调，以及与粒长呈负相关的基因（）下调。同时，虽然白米中的总淀粉含量未因氢纳米气泡水而改变，但直链淀粉含量降低了31.6%，这与直链淀粉代谢基因转录本的变化平行。特别是，白米中的镉积累显著减少，降至对照组的52%。这一现象与负责镉进入植物的转运蛋白基因的差异表达密切相关，包括下调的（）、（和）以及（），以及负责减少籽粒中镉积累的（）下调。这项研究清楚地表明，施用分子氢可能是提高水稻田间产量和稻谷品质的有效途径。
