Li Longna, Yin Qianlan, Zhang Tong, Cheng Pengfei, Xu Sheng, Shen Wenbiao
Laboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.
Plants (Basel). 2021 Aug 12;10(8):1662. doi: 10.3390/plants10081662.
The short vase life of cut flowers limits their commercial value. To ameliorate this practical problem, this study investigated the effect of hydrogen nanobubble water (HNW) on delaying senescence of cut carnation flowers ( L.). It was observed that HNW had properties of higher concentration and residence time for the dissolved hydrogen gas in comparison with conventional hydrogen-rich water (HRW). Meanwhile, application of 5% HNW significantly prolonged the vase life of cut carnation flowers compared with distilled water, other doses of HNW (including 1%, 10%, and 50%), and 10% HRW, which corresponded with the alleviation of fresh weight and water content loss, increased electrolyte leakage, oxidative damage, and cell death in petals. Further study showed that the increasing trend with respect to the activities of nucleases (including DNase and RNase) and protease during vase life period was inhibited by 5% HNW. The results indicated that HNW delayed petal senescence of cut carnation flowers through reducing reactive oxygen species accumulation and initial activities of senescence-associated enzymes. These findings may provide a basic framework for the application of HNW for postharvest preservation of agricultural products.
切花较短的瓶插寿命限制了它们的商业价值。为改善这一实际问题，本研究调查了纳米氢气泡水（HNW）对延缓香石竹切花衰老的影响。据观察，与传统富氢水（HRW）相比，纳米氢气泡水具有更高的溶解氢气浓度和停留时间。同时，与蒸馏水、其他剂量的纳米氢气泡水（包括1%、10%和50%）以及10%的富氢水相比，施用5%的纳米氢气泡水显著延长了香石竹切花的瓶插寿命，这与减轻鲜重和水分含量损失、减少花瓣中的电解质渗漏、氧化损伤和细胞死亡相对应。进一步研究表明，5%的纳米氢气泡水抑制了瓶插寿命期间核酸酶（包括脱氧核糖核酸酶和核糖核酸酶）和蛋白酶活性的上升趋势。结果表明，纳米氢气泡水通过减少活性氧积累和衰老相关酶的初始活性来延缓香石竹切花花瓣衰老。这些发现可能为纳米氢气泡水在农产品采后保鲜中的应用提供一个基本框架。
