Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, 510640, PR China.
College of Horticulture, South China Agricultural University, Guangzhou, 510642, PR China.
Biomed Pharmacother. 2020 May;125:109893. doi: 10.1016/j.biopha.2020.109893. Epub 2020 Jan 28.
Black tea and green tea were produced via different processing techniques from the same tea leave variety. Then, biochemical components of the two water extracts were analysed to study cell apoptosis, migration and invasion of HepG2 cells induced by black tea and green tea.
The monomer components of the black tea and green tea extracts were analysed by colorimetry and HPLC, with MTT assay and colony formation assays used to assess cell proliferation and viability. The effects of black tea and green tea on apoptosis of HepG2 cells were verified by flow cytometry, with wound healing and Transwell experiments used to detect cell invasion and metastasis. The expression of PI3K/Akt signalling and apoptosis-related proteins as well as epithelial-mesenchymal transition (EMT) regulatory factor in HepG2 cells were determined by western blotting after black tea and green tea treatment.
Black tea and green tea extracts demonstrated different degrees of inhibition of cell migration and invasion, with green tea inducing more HepG2 cell apoptosis. In addition, green tea and black tea extracts inhibited the growth of HepG2 cells and induced apoptosis via PI3K/Akt, and inhibited cell migration and invasion through the MMPs signalling pathway. This study revealed the effects of fermented (black tea) and non-fermented tea (green tea) on liver cancer cells, providing a basis for the investigation of tea extracts for their anti-tumour potential.
红茶和绿茶是由同一种茶叶通过不同的加工工艺制成的。然后，分析这两种水提取物的生化成分，研究红茶和绿茶对 HepG2 细胞凋亡、迁移和侵袭的影响。
采用比色法和 HPLC 分析红茶和绿茶提取物的单体成分，用 MTT 法和集落形成实验评估细胞增殖和活力。通过流式细胞术验证红茶和绿茶对 HepG2 细胞凋亡的影响，用划痕愈合和 Transwell 实验检测细胞侵袭和转移。用 Western blot 检测红茶和绿茶处理后 HepG2 细胞中 PI3K/Akt 信号转导和凋亡相关蛋白以及上皮间质转化（EMT）调节因子的表达。
红茶和绿茶提取物对细胞迁移和侵袭的抑制程度不同，绿茶诱导 HepG2 细胞凋亡的作用更强。此外，红茶和绿茶提取物通过 PI3K/Akt 抑制 HepG2 细胞生长并诱导细胞凋亡，通过 MMPs 信号通路抑制细胞迁移和侵袭。本研究揭示了发酵茶（红茶）和未发酵茶（绿茶）对肝癌细胞的影响，为研究茶提取物的抗肿瘤潜力提供了依据。
