Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, China.
College of Environment and Resource, Guangxi Normal University, Guilin, China.
Planta Med. 2020 Sep;86(13-14):983-987. doi: 10.1055/a-1102-1168. Epub 2020 Jan 31.
Endophytic fungi can produce beneficial active components during symbiosis with host plants. Fifteen purified endophytic fungal strains were isolated and screened from root, stem, leaf, and fruit of the Chinese medicine food homologous plant . These strains were fermented, and then the fermented solution was extracted using acetonitrile. The extracts were analyzed by high performance liquid chromatography (HPLC) and mass spectrum (MS), using standard mogroside V as control. The results showed that strain LHG-F5 and strain LHG-L4 could produce mogroside V with output of 3.814 µg/mL and 2.675 µg/mL, respectively. By morphological observation together with similarity of internal transcribed spacer (ITS) sequence by Clustan X method, LHG-F5 and LHG-L4 were classified as and , respectively.
内生真菌在与宿主植物共生时可以产生有益的活性成分。从中药同源植物的根、茎、叶和果实中分离筛选出 15 株纯化内生真菌菌株。对这些菌株进行发酵，然后用乙腈提取发酵液。采用高效液相色谱（HPLC）和质谱（MS）法，以标准罗汉果苷 V 为对照进行分析。结果表明，菌株 LHG-F5 和菌株 LHG-L4 分别能产生 3.814μg/mL 和 2.675μg/mL 的罗汉果苷 V。通过形态观察和 Clustan X 方法的 ITS 序列相似性分析，将 LHG-F5 和 LHG-L4 分别归类为 和 。
