State Key Laboratory of Genetic Engineering, Institute of Genetics, Institute of Plant Biology, School of Life Science, Fudan University, Shanghai 200433, China.
State Key Laboratory of Genetic Engineering, Institute of Genetics, Institute of Plant Biology, School of Life Science, Fudan University, Shanghai 200433, China; Institute of Biothermal Science and Technology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Plant Sci. 2020 Mar;292:110373. doi: 10.1016/j.plantsci.2019.110373. Epub 2019 Dec 13.
SnRK2 (sucrose non-fermenting 1-related protein kinases 2) protein kinase family involves in several abiotic stress response in plants. Although the regulatory mechanism of SnRK2 have been well demonstrated in Arabidopsis thaliana, their functions in rice are still largely unknown. Here, we report a SnRK2 family gene, OsSAPK8, can be strongly induced by abiotic stresses, including low-temperature, drought and high salt stress. The ossapk8 mutants showed lower tolerance to low-temperature, high salinity and drought stresses at the vegetative stages. Moreover, the expressions of marker genes for those abiotic stresses, e.g. OsDREB1, OsDREB2, OsNCED and OsRAB21, were downregulated in the ossapk8 mutants. We further confirmed that the yield was reduced in ossapk8 mutant lines compared with the wild type. Our results provide evidence for OsSAPK8 acting as a positive regulator in cold, drought, and salt stress responses.
SnRK2（蔗糖非发酵 1 相关蛋白激酶 2）蛋白激酶家族参与植物的多种非生物胁迫响应。尽管 SnRK2 的调节机制在拟南芥中已得到很好的证明，但它们在水稻中的功能仍知之甚少。在这里，我们报告了一个 SnRK2 家族基因 OsSAPK8，它可以被非生物胁迫强烈诱导，包括低温、干旱和高盐胁迫。ossapk8 突变体在营养生长阶段对低温、高盐和干旱胁迫的耐受性较低。此外，那些非生物胁迫的标记基因，如 OsDREB1、OsDREB2、OsNCED 和 OsRAB21 的表达在 ossapk8 突变体中下调。我们进一步证实，ossapk8 突变体系的产量与野生型相比降低。我们的结果为 OsSAPK8 在冷、干旱和盐胁迫响应中作为正调节剂提供了证据。
