Zan Xiaofei, Zhou Zhanmei, Wan Jiale, Chen Hao, Zhu Jiali, Xu Haoran, Zhang Jia, Li Xiaohong, Gao Xiaoling, Chen Rongjun, Huang Zhengjian, Xu Zhengjun, Li Lihua
Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, People's Republic of China.
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, People's Republic of China.
Rice (N Y). 2023 Jul 19;16(1):31. doi: 10.1186/s12284-023-00647-y.
Haloacid dehalogenase-like hydrolase (HAD) superfamily have been shown to get involved in plant growth and abiotic stress response. Although the various functions and regulatory mechanism of HAD superfamily have been well demonstrated, we know little about the function of this family in conferring abiotic stress tolerance to rice. Here, we report OsHAD3, a HAD superfamily member, could affect drought tolerance of rice. Under drought stress, overexpression of OsHAD3 increases the accumulation of reactive oxygen species and malondialdehyde than wild type. OsHAD3-overexpression lines decreased but antisense-expression lines increased the roots length under drought stress and the transcription levels of many well-known stress-related genes were also changed in plants with different genotypes. Furthermore, overexpression of OsHAD3 also decreases the oxidative tolerance. Our results suggest that overexpression of OsHAD3 could decrease the drought tolerance of rice and provide a new strategy for improving drought tolerance in rice.
卤代酸脱卤酶样水解酶（HAD）超家族已被证明参与植物生长和非生物胁迫响应。尽管HAD超家族的各种功能和调控机制已得到充分证明，但我们对该家族在赋予水稻非生物胁迫耐受性方面的功能了解甚少。在此，我们报道了HAD超家族成员OsHAD3会影响水稻的耐旱性。在干旱胁迫下，与野生型相比，OsHAD3的过表达增加了活性氧和丙二醛的积累。在干旱胁迫下，OsHAD3过表达系的根长缩短，而反义表达系的根长增加，并且在不同基因型的植物中，许多著名的胁迫相关基因的转录水平也发生了变化。此外，OsHAD3的过表达还降低了氧化耐受性。我们的结果表明，OsHAD3的过表达会降低水稻的耐旱性，并为提高水稻耐旱性提供了新策略。
