Agronomy College, Sichuan Agricultural University, Chengdu 611130, China.
Genes (Basel). 2023 Oct 9;14(10):1918. doi: 10.3390/genes14101918.
The basic (region) leucine zippers (bZIPs) are evolutionarily conserved transcription factors widely distributed in eukaryotic organisms. In plants, they are not only involved in growth and development, defense and stress responses and regulation of physiological processes but also play a pivotal role in regulating secondary metabolism. To explore the function related to the bZIP gene family in Bertoni, we identified 105 genes at the genome-wide level and classified them into 12 subfamilies using bioinformation methods. Three main classes of -acting elements were found in the promoter regions, including development-related elements, defense and stress-responsive elements and phytohormone-responsive elements. Through protein-protein interaction network of 105 SrbZIP proteins, SrbZIP proteins were mainly classified into four major categories: ABF2/ABF4/ABI5 (SrbZIP51/SrbZIP38/SrbZIP7), involved in phytohormone signaling, GBF1/GBF3/GBF4 (SrbZIP29/SrbZIP63/SrbZIP60) involved in environmental signaling, AREB3 (SrbZIP88), PAN (SrbZIP12), TGA1 (SrbZIP69), TGA4 (SrbZIP82), TGA7 (SrbZIP31), TGA9 (SrbZIP95), TGA10 (SrbZIP79) and HY5 (SrbZIP96) involved in cryptochrome signaling, and FD (SrbZIP72) promoted flowering. The transcriptomic data showed that genes were differentially expressed in six cultivars ('023', '110', 'B1188', '11-14', 'GP' and 'GX'). Moreover, the expression levels of selected 15 genes in response to light, abiotic stress (low temperature, salt and drought), phytohormones (methyl jasmonate, gibberellic acid and salicylic acid) treatment and in different tissues were analyzed utilizing qRT-PCR. Some genes were further identified to be highly induced by factors affecting glycoside synthesis. Among them, three genes (, and ) were predicted to be related to stress-responsive terpenoid synthesis in . The protein-protein interaction network expanded the potential functions of genes. This study firstly provided the comprehensive genome-wide report of the gene family, laying a foundation for further research on the evolution, function and regulatory role of the bZIP gene family in terpenoid synthesis in .
该基本（区域）亮氨酸拉链（bZIP）是进化上保守的转录因子，广泛分布于真核生物中。在植物中，它们不仅参与生长发育、防御和应激反应以及生理过程的调节，而且在调节次生代谢物方面也起着关键作用。为了探讨 Bertoni 中 bZIP 基因家族的功能，我们在全基因组水平上鉴定了 105 个基因，并使用生物信息学方法将它们分为 12 个亚家族。在启动子区域中发现了 3 种主要的 - 作用元件，包括与发育相关的元件、防御和应激响应元件以及植物激素响应元件。通过 105 个 SrbZIP 蛋白的蛋白质-蛋白质相互作用网络，SrbZIP 蛋白主要分为四大类：ABF2/ABF4/ABI5（SrbZIP51/SrbZIP38/SrbZIP7），参与植物激素信号转导；GBF1/GBF3/GBF4（SrbZIP29/SrbZIP63/SrbZIP60），参与环境信号转导；AREB3（SrbZIP88）、PAN（SrbZIP12）、TGA1（SrbZIP69）、TGA4（SrbZIP82）、TGA7（SrbZIP31）、TGA9（SrbZIP95）、TGA10（SrbZIP79）和 HY5（SrbZIP96），参与隐花色素信号转导；FD（SrbZIP72）促进开花。转录组数据显示，6 个品种（'023'、'110'、'B1188'、'11-14'、'GP'和'GX'）中有 141 个基因差异表达。此外，利用 qRT-PCR 分析了 15 个选定基因对光、非生物胁迫（低温、盐和干旱）、植物激素（茉莉酸甲酯、赤霉素和水杨酸）处理以及不同组织的表达水平。进一步鉴定了一些基因对影响糖苷合成的因子高度诱导。其中，三个基因（、和）被预测与 Bertoni 中应激响应萜类合成有关。蛋白质-蛋白质相互作用网络扩展了基因的潜在功能。本研究首次提供了 bZIP 基因家族的全基因组综合报告，为进一步研究 bZIP 基因家族在萜类合成中的进化、功能和调控作用奠定了基础。
