Feng Xue, Meng Quan, Zeng Jianbin, Yu Qian, Xu Dengan, Dai Xuehuan, Ge Lei, Ma Wujun, Liu Wenxing
College of Agronomy, Qingdao Agricultural University, Qingdao, China.
State Agricultural Biotechnology Centre, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia.
Front Plant Sci. 2022 Dec 22;13:1087839. doi: 10.3389/fpls.2022.1087839. eCollection 2022.
Protein kinases play an important role in plants in response to environmental changes through signal transduction. As a large family of protein kinases, sucrose non-fermenting-1 (SNF1)-related kinases (SnRKs) were found and functionally verified in many plants. Nevertheless, little is known about the family of .
Evolutionary relationships, chromosome locations, gene structures, conserved motifs, and cis-elements in promoter regions were systematically analyzed. Besides, tissue-specific and stress-induced expression patterns of were determined. Finally, functional regulatory networks between and other proteins or miRNAs were constructed.
In total, 60 genes located on 10 chromosomes were discovered in maize. were classified into three subfamilies (, , and ), consisting of 4, 14, and 42 genes, respectively. Gene structure analysis showed that 33 of the 42 genes contained only one exon. Most genes contained at least one ABRE, MBS, and LTR cis-element and a few genes had AuxRR-core, P-box, MBSI, and SARE ciselements in their promoter regions. The Ka:Ks ratio of 22 paralogous gene pairs revealed that the gene family had experienced a purifying selection. Meanwhile, we analyzed the expression profiles of , and they exhibited significant differences in various tissues and abiotic stresses. In addition, A total of eight ZmPP2Cs, which can interact with proteins, and 46 miRNAs, which can target 24 , were identified. Generally, these results provide valuable information for further function verification of , and improve our understanding of the role of in the climate resilience of maize.
蛋白激酶在植物通过信号转导响应环境变化中发挥着重要作用。作为一大类蛋白激酶，蔗糖非发酵-1（SNF1）相关激酶（SnRKs）已在许多植物中被发现并进行了功能验证。然而，关于该家族的情况却知之甚少。
系统分析了进化关系、染色体定位、基因结构、保守基序以及启动子区域的顺式作用元件。此外，还确定了其组织特异性和胁迫诱导表达模式。最后，构建了与其他蛋白质或miRNA之间的功能调控网络。
在玉米中总共发现了60个位于10条染色体上的基因。它们被分为三个亚家族（、和），分别由4个、14个和42个基因组成。基因结构分析表明，42个基因中的33个仅包含一个外显子。大多数基因在其启动子区域至少包含一个ABRE、MBS和LTR顺式作用元件，少数基因具有AuxRR-core、P-box、MBSI和SARE顺式作用元件。22对旁系同源基因对的Ka:Ks比值表明该基因家族经历了纯化选择。同时，我们分析了的表达谱，它们在不同组织和非生物胁迫下表现出显著差异。此外，共鉴定出8个可与蛋白相互作用的ZmPP2C以及46个可靶向24个的miRNA。总体而言，这些结果为进一步验证的功能提供了有价值的信息，并增进了我们对在玉米气候适应性中作用的理解。
