Department of Molecular Biology and Genetics, Faculty of Science, Izmir Institute of Technology, Izmir 35430, Turkey.
MULTI Tarım Seed Company, Antalya 07112, Turkey.
Plant Sci. 2020 Mar;292:110393. doi: 10.1016/j.plantsci.2019.110393. Epub 2019 Dec 27.
The nutritional value of a crop lies not only in its protein, lipid, and sugar content but also involves compounds such as the antioxidants lycopene, β-carotene and vitamin C. In the present study, wild tomato Solanum pimpinellifolium LA 1589 was assessed for its potential to improve antioxidant content. This wild species was found to be a good source of alleles for increasing β-carotene, lycopene, vitamin C and vitamin E contents in cultivated tomato. Characterization of an LA 1589 interspecific inbred backcross line (IBL) mapping population revealed many individuals with transgressive segregation for the antioxidants confirming the usefulness of this wild species for breeding of these traits. Molecular markers were used to identify QTLs for the metabolites in the IBL population. In total, 64 QTLs were identified for the antioxidants and their locations were compared to the map positions of previously identified QTLs for confirmation. Four (57 %) of the carotenoid QTLs, four (36 %) of the vitamin QTLs, and 11 (25 %) of the phenolic acid QTLs were supported by previous studies. Furthermore, several potential candidate genes were identified for vitamins C and E and phenolic acids loci. These candidate genes might be used as markers in breeding programs to increase tomato's antioxidant content.
作物的营养价值不仅在于其蛋白质、脂肪和糖分含量，还涉及到抗氧化剂番茄红素、β-胡萝卜素和维生素 C 等化合物。在本研究中，评估了野生番茄 Solanum pimpinellifolium LA 1589 提高抗氧化剂含量的潜力。该野生种是增加栽培番茄中β-胡萝卜素、番茄红素、维生素 C 和维生素 E 含量的等位基因的良好来源。对 LA 1589 种间近交系回交群体（IBL）的作图群体进行了表征，发现许多个体对抗氧化剂表现出超亲分离，证实了该野生种在这些性状的育种中的有用性。利用分子标记鉴定了 IBL 群体中代谢物的 QTL。共鉴定到 64 个与抗氧化剂相关的 QTL，其位置与先前鉴定到的用于确认的 QTL 的图谱位置进行了比较。4 个（57%）类胡萝卜素 QTL、4 个（36%）维生素 QTL 和 11 个（25%）酚酸 QTL 得到了先前研究的支持。此外，还鉴定到了维生素 C 和 E 以及酚酸位点的几个潜在候选基因。这些候选基因可用于育种计划，以提高番茄的抗氧化剂含量。
