Center of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, 502324, India.
Center of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, 502324, India.
Curr Opin Plant Biol. 2020 Aug;56:190-196. doi: 10.1016/j.pbi.2019.12.004. Epub 2020 Jan 28.
Here we propose a 5G breeding approach for bringing much-needed disruptive changes to crop improvement. These 5Gs are Genome assembly, Germplasm characterization, Gene function identification, Genomic breeding (GB), and Gene editing (GE). In our view, it is important to have genome assemblies available for each crop and a deep collection of germplasm characterized at sequencing and agronomic levels for identification of marker-trait associations and superior haplotypes. Systems biology and sequencing-based mapping approaches can be used to identify genes involved in pathways leading to the expression of a trait, thereby providing diagnostic markers for target traits. These genes, markers, haplotypes, and genome-wide sequencing data may be utilized in GB and GE methodologies in combination with a rapid cycle breeding strategy.
在这里，我们提出了一种 5G 培育方法，旨在为作物改良带来急需的颠覆性变革。这 5G 分别是基因组组装、种质资源特征描述、基因功能鉴定、基因组育种（GB）和基因编辑（GE）。我们认为，为每个作物提供基因组组装，以及对大量种质资源进行测序和农艺水平的特征描述，以鉴定标记-性状关联和优良单倍型，这一点很重要。系统生物学和基于测序的作图方法可用于鉴定参与导致性状表达的途径的基因，从而为目标性状提供诊断标记。这些基因、标记、单倍型和全基因组测序数据可与快速循环育种策略一起用于 GB 和 GE 方法中。
