Department of Environment and Health, School of Public Health, Shandong University, Jinan, 250012, China.
Department of Clinical and Experimental Medicine, Linköping University, 581 85, Linköping, Sweden; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Environ Pollut. 2020 May;260:114041. doi: 10.1016/j.envpol.2020.114041. Epub 2020 Jan 22.
Infections caused by carbapenem-resistant Enterobacteriaceae are a growing concern worldwide. Raoultella ornithinolytica is a species in the Enterobacteriaceae family which can cause hospital-acquired infections and is sporadically reported as carbapenem-resistant from human and environmental sources. In this study, we firstly report on an NDM-1-producing R. ornithinolytica, Rao166, isolated from drinking water in an animal cultivation area in China. In addition to carbapenem-resistance, Rao166 was resistant to several other antibiotics including gentamicin, sulfamethoxazole-trimethoprim, tetracycline and fosfomycin. Rao166 carried a novel IncFIC-type megaplasmid, 382,325 bp in length (pRAO166a). A multidrug resistance region, 60,600 bp in length, was identified in the plasmid containing an aac(3)-IId-like gene, aac(6')-Ib-cr, bla, bla, bla, bla, bla, qnrB4, catB3, arr-3, sul1, and tet(D). Results from virulence assays implied that Rao166 has considerable pathogenic potential. Although pRAO166a was found to be non-transmissible, dissemination of the NDM-1 producing strain may occur from well water to humans or animals through cross-contamination during food preparation or directly via drinking water, and potentially lead to difficult-to-treat infections. Thus, contamination of well water by this carbapenem-resistant and presumptively virulent strain of R. ornithinolytica should be considered a potential public health risk.
产碳青霉烯酶肠杆菌科细菌引起的感染是全球日益关注的问题。鸟氨酸罗伊氏菌是肠杆菌科的一个物种，可引起医院获得性感染，并且偶尔有人类和环境来源的碳青霉烯类耐药报告。在这项研究中，我们首次报道了一株产 NDM-1 的鸟氨酸罗伊氏菌，Rao166，从中国动物养殖区的饮用水中分离得到。除了碳青霉烯类耐药性外，Rao166 还对其他几种抗生素耐药，包括庆大霉素、磺胺甲恶唑-甲氧苄啶、四环素和磷霉素。Rao166 携带一种新型 IncFIC 型大型质粒，长 382,325bp（pRAO166a）。在质粒中鉴定出一个长 60,600bp 的多药耐药区，包含一个 aac(3)-IId-like 基因、aac(6')-Ib-cr、bla、bla、bla、bla、bla、qnrB4、catB3、arr-3、sul1 和 tet(D)。毒力试验结果表明，Rao166 具有相当大的致病潜力。虽然未发现 pRAO166a 可传播，但由于在食品制备过程中发生交叉污染或直接通过饮用水，耐碳青霉烯类且可能具有潜在毒力的菌株可能会从井水传播到人类或动物，从而导致难以治疗的感染。因此，应将这种耐碳青霉烯类和可能具有潜在毒力的鸟氨酸罗伊氏菌对井水的污染视为潜在的公共卫生风险。
