Gibbon Marjorie J, Couto Natacha, David Sophia, Barden Ruth, Standerwick Richard, Jagadeesan Kishore, Birkwood Hollie, Dulyayangkul Punyawee, Avison Matthew B, Kannan Andrew, Kibbey Dan, Craft Tim, Habib Samia, Thorpe Harry A, Corander Jukka, Kasprzyk-Hordern Barbara, Feil Edward J
The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Bath, UK.
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Microb Genom. 2021 Mar;7(3). doi: 10.1099/mgen.0.000509. Epub 2021 Jan 8.
species occupy a wide range of environmental and animal niches, and occasionally cause opportunistic infections that are resistant to multiple antibiotics. In particular, () has gained notoriety as a major nosocomial pathogen, due principally to the rise in non-susceptibility to carbapenems and other beta-lactam antibiotics. Whilst it has been proposed that the urban water cycle facilitates transmission of pathogens between clinical settings and the environment, the level of risk posed by resistant strains in hospital wastewater remains unclear. We used whole genome sequencing (WGS) to compare species in contemporaneous samples of wastewater from an English hospital and influent to the associated wastewater treatment plant (WWTP). As we aimed to characterize representative samples of communities, we did not actively select for antibiotic resistance (other than for ampicillin), nor for specific species. Two species, and . () (), were of equal dominance in the hospital wastewater, and four other species were present in low abundance in this sample. In contrast, despite being the species most closely associated with healthcare settings, was the dominant species within the WWTP influent. In total, 29 % of all isolates harboured the gene on a pOXA-48-like plasmid, and these isolates were almost exclusively recovered from the hospital wastewater. This gene was far more common in (68 % of isolates) than in (3.4 % of isolates). In general plasmid-borne, but not chromosomal, resistance genes were significantly enriched in the hospital wastewater sample. These data implicate hospital wastewater as an important reservoir for antibiotic-resistant , and point to an unsuspected role of species within the group in the maintenance and dissemination of plasmid-borne . This article contains data hosted by Microreact.
该物种占据广泛的环境和动物生态位，偶尔会引发对多种抗生素耐药的机会性感染。特别是，（）作为主要的医院病原体声名狼藉，主要原因是对碳青霉烯类和其他β-内酰胺类抗生素的耐药性增加。虽然有人提出城市水循环促进了病原体在临床环境与环境之间的传播，但医院废水中耐药菌株所带来的风险水平仍不明确。我们使用全基因组测序（WGS）来比较一家英国医院同期废水样本以及相关污水处理厂（WWTP）进水样本中的（）物种。由于我们旨在对（）群落的代表性样本进行特征描述，所以我们没有主动选择耐药性（除了氨苄青霉素），也没有选择特定的（）物种。两种物种，（）和（）（），在医院废水中占同等优势，该样本中还存在其他四种低丰度的（）物种。相比之下，尽管（）是与医疗环境关联最紧密的物种，但它是污水处理厂进水样本中的优势物种。总体而言，所有分离株中有29%在类pOXA - 48质粒上携带（）基因，这些分离株几乎都从医院废水中分离得到。该基因在（）中更为常见（68%的分离株），而在（）中较少见（3.4%的分离株）。一般来说，质粒携带而非染色体携带的耐药基因在医院废水样本中显著富集。这些数据表明医院废水是耐药（）的重要储存库，并指出（）组内物种在质粒携带（）的维持和传播中存在未被怀疑的作用。本文包含由Microreact托管的数据。
