Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Bioresour Technol. 2020 Apr;302:122874. doi: 10.1016/j.biortech.2020.122874. Epub 2020 Jan 23.
Pretreatment of microalgal biomass possessing rigid cell wall is a critical step for enhancing the efficiency of microalgal biorefinery. However, the conventional pretreatment processes suffer the drawbacks of complex processing steps, long processing time, low conversion efficiency and high processing costs. This significantly hinders the industrial applicability of microalgal biorefinery. The innovative electricity-aid pretreatment techniques serve as a promising processing tool to extensively enhance the release of intracellular substances from microalgae. In this review, application of electric field-based techniques and recent advances of using electrical pretreatments on microalgae cell focusing on pulsed electric field, electrolysis, high voltage electrical discharges and moderate electric field are reviewed. In addition, the emerging techniques integrating electrolysis with liquid biphasic flotation process as promising downstream approach is discussed. This review delivers broad knowledge of the present significance of the application of these methods focusing on the development of electric assisted biomolecules extraction from microalgae.
预处理具有刚性细胞壁的微藻生物质是提高微藻生物炼制效率的关键步骤。然而，传统的预处理工艺存在处理步骤复杂、处理时间长、转化率低和处理成本高的缺点。这极大地阻碍了微藻生物炼制的工业适用性。创新的电力辅助预处理技术是一种很有前途的处理工具，可以广泛提高微藻细胞内物质的释放效率。在本文综述中，介绍了基于电场的技术的应用，以及最近在微藻细胞上使用电预处理的进展，重点介绍了脉冲电场、电解、高压放电和中等电场。此外，还讨论了将电解与液-液两相浮选工艺相结合的新兴技术，作为一种很有前途的下游方法。本综述提供了广泛的知识，介绍了这些方法在从微藻中提取生物分子方面的应用现状，重点介绍了电辅助方法的发展。
