Azmi Abdul Azim Bin, Chew Kit Wayne, Chia Wen Yi, Mubashir Muhammad, Sankaran Revathy, Lam Man Kee, Lim Jun Wei, Ho Yeek-Chia, Show Pau Loke
Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
School of Energy and Chemical Engineering, Xiamen University Malaysia, Jalan Sunsuria, Bandar Sunsuria, 43900 Sepang, Selangor, Malaysia; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China.
Bioresour Technol. 2021 Aug;333:125197. doi: 10.1016/j.biortech.2021.125197. Epub 2021 Apr 21.
The work aimed to study the potential in producing a system with high microalgal protein recovery and separation by utilizing a one-step or integrated downstream process. This in turn enables green biorefinery of protein, contributing to circular bioeconomy whereby less energy, labor, and cost are required for the process. By utilizing electric three phase partitioning flotation system, high protein recovery yield, R of 99.42 ± 0.52% and high separation efficiency, E of 52.72 ± 0.40% system was developed. Scaling up also showed high protein recovery yield with R value of 89.13 ± 1.56%. Total processing duration (extraction, separation, and purification) was also significantly reduced to 10 min. This system showed remarkable potential in reducing processing time, alternatively cost of production, benefiting microalgal downstream processing. Concisely, through this system, microalgal bioprocessing will no longer be complex allowing a wide array of potentials for further studies in this field.
这项工作旨在研究通过利用一步法或集成下游工艺来生产具有高微藻蛋白回收率和分离率的系统的潜力。这反过来又能实现蛋白质的绿色生物精炼，有助于循环生物经济，从而在该过程中减少能源、劳动力和成本。通过利用三相电分离浮选系统，开发出了蛋白质回收率高达99.42±0.52%、分离效率高达52.72±0.40%的系统。放大实验也显示出高蛋白质回收率，R值为89.13±1.56%。总处理时间（提取、分离和纯化）也显著缩短至10分钟。该系统在减少处理时间以及生产成本方面显示出显著潜力，有利于微藻下游加工。简而言之，通过该系统，微藻生物加工将不再复杂，为该领域的进一步研究带来了广泛的潜力。
