LI-COR Biosciences, 4647 Superior Street, Lincoln, NE, 68504, USA.
LI-COR Biosciences, 4647 Superior Street, Lincoln, NE, 68504, USA.
Anal Biochem. 2020 Mar 15;593:113608. doi: 10.1016/j.ab.2020.113608. Epub 2020 Jan 31.
Attaining true quantitative data from WB requires that all the players involved in the procedure are quality controlled including the user. Appropriate protein extraction method, electrophoresis, and transfer of proteins, immunodetection of blotted protein by antibodies, and the ultimate step of imaging and analyzing the data is nothing short of a symphony. Like with any other technology in life-sciences research, Western blotting can produce erroneous and irreproducible data. We provide a systematic approach to generate quantitative data from Western blot experiments that incorporates critical validation steps to identify and minimize sources of error and variability throughout the Western blot process.
从 WB 获得真正的定量数据要求参与该过程的所有人员（包括用户）都经过质量控制。适当的蛋白质提取方法、电泳和蛋白质转移、抗体对印迹蛋白的免疫检测，以及最终的成像和数据分析步骤，简直就是一场交响乐。与生命科学研究中的任何其他技术一样，Western blot 也可能产生错误和不可重复的数据。我们提供了一种从 Western blot 实验中生成定量数据的系统方法，该方法纳入了关键的验证步骤，以识别和最小化 Western blot 过程中误差和可变性的来源。
