细胞内谷胱甘肽水平与乏氧细胞放射敏感性之间的关系。

Relationship between intracellular GSH levels and hypoxic cell radiosensitivity.

作者信息

Astor M B, Anderson M E, Meister A

机构信息

Columbia College of Physicians and Surgeons, Radiological Research Laboratory, New York, New York 10032.

出版信息

Pharmacol Ther. 1988;39(1-3):115-21. doi: 10.1016/0163-7258(88)90049-6.

DOI:10.1016/0163-7258(88)90049-6

PMID:3200883

Abstract

The present studies show the following: (a) The plating density of cells (V79 and GM3877) affects the experimentally determined OER values. (b) Fibroblasts depleted of cysteine and GSH are more sensitive to radiation under hypoxic conditions (under aerobic conditions the absence of cysteine or GSH has no detectable effect on radiation sensitivity). (c) Addition of GEE to V79 cells (previously depleted of GSH) leads to increased intracellular GSH levels and protects the cells against radiation under hypoxic conditions.

摘要

目前的研究表明如下

(a) 细胞（V79和GM3877）的接种密度会影响实验测定的氧增强比（OER）值。(b) 缺乏半胱氨酸和谷胱甘肽（GSH）的成纤维细胞在低氧条件下对辐射更敏感（在有氧条件下，缺乏半胱氨酸或GSH对辐射敏感性没有可检测到的影响）。(c) 向V79细胞（先前已耗尽GSH）中添加GEE会导致细胞内GSH水平升高，并在低氧条件下保护细胞免受辐射。