Department of Environmental Science, Aarhus University, RISØ Campus, Roskilde, 4000, Denmark.
Department of Biology, University of Copenhagen, DK-2100, Copenhagen, Denmark.
FEMS Microbiol Ecol. 2020 Mar 1;96(3). doi: 10.1093/femsec/fiaa016.
Recycling of wood ash from energy production may counteract soil acidification and return essential nutrients to soils. However, wood ash amendment affects soil physicochemical parameters that control composition and functional expression of the soil microbial community. Here, we applied total RNA sequencing to simultaneously assess the impact of wood ash amendment on the active soil microbial communities and the expression of functional genes from all microbial taxa. Wood ash significantly affected the taxonomic (rRNA) as well as functional (mRNA) profiles of both agricultural and forest soil. Increase in pH, electrical conductivity, dissolved organic carbon and phosphate were the most important physicochemical drivers for the observed changes. Wood ash amendment increased the relative abundance of the copiotrophic groups Chitinonophagaceae (Bacteroidetes) and Rhizobiales (Alphaproteobacteria) and resulted in higher expression of genes involved in metabolism and cell growth. Finally, total RNA sequencing allowed us to show that some groups of bacterial feeding protozoa increased concomitantly to the enhanced bacterial growth, which shows their pivotal role in the regulation of bacterial abundance in soil.
从能源生产中回收的草木灰可能会中和土壤酸化，并向土壤中归还基本养分。然而，草木灰的添加会影响控制土壤微生物群落组成和功能表达的土壤理化参数。在这里，我们应用总 RNA 测序同时评估草木灰添加对活性土壤微生物群落和所有微生物分类群功能基因表达的影响。草木灰显著影响了农业和森林土壤的分类（rRNA）和功能（mRNA）谱。pH 值、电导率、溶解有机碳和磷酸盐的增加是观察到的变化的最重要的理化驱动因素。草木灰的添加增加了富营养菌的相对丰度，如拟杆菌科（Bacteroidetes）和根瘤菌目（Alphaproteobacteria），并导致与代谢和细胞生长相关的基因表达增加。最后，总 RNA 测序使我们能够表明，一些细菌吞噬原生动物的群体与增强的细菌生长同时增加，这表明它们在调节土壤中细菌丰度方面发挥着关键作用。
