Schubiger Carla B, Hoang Kelli H T, Häse Claudia C
Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon State University, Corvallis, OR, 97331, USA.
College of Pharmacy, Oregon State University, Corvallis, OR, 97331, USA.
J Genet Eng Biotechnol. 2020 Feb 3;18(1):4. doi: 10.1186/s43141-020-0019-y.
Pseudomonas aeruginosa is a bacterial pathogen that can cause grave and sometimes chronic infections in patients with weakened immune systems and cystic fibrosis. It is expected that sodium/proton transporters in the cellular membrane are crucial for the organism's survival and growth under certain conditions, since many cellular processes rely on the maintenance of Na and H transmembrane gradients.
This study focused on the role of the primary and secondary proton and/or sodium pumps Mrp, Nuo, NhaB, NhaP, and NQR for growth, biofilm formation, and swarming motility in P. aeruginosa. Using mutants with gene deletions, we investigated the impact of each sodium pump's absence on the overall growth, biofilm formation, motility, and weak acid tolerance of the organism. We found that the absence of some, but not all, of the sodium pumps have a deleterious effect on the different phenotypes of P. aeruginosa.
The absence of the Mrp sodium/proton antiporter was clearly important in the organism's ability to survive and function in environments of higher pH and sodium concentrations, while the absence of Complex I, which is encoded by the nuo genes, had some consistent impact on the organism's growth regardless of the pH and sodium concentration of the environment.
铜绿假单胞菌是一种细菌病原体，可在免疫系统较弱的患者和囊性纤维化患者中引起严重的、有时是慢性的感染。由于许多细胞过程依赖于钠和氢跨膜梯度的维持，因此细胞膜中的钠/质子转运蛋白在特定条件下对该生物体的生存和生长至关重要。
本研究聚焦于初级和次级质子和/或钠泵Mrp、Nuo、NhaB、NhaP和NQR在铜绿假单胞菌生长、生物膜形成和群体运动中的作用。使用基因缺失突变体，我们研究了每个钠泵缺失对该生物体整体生长、生物膜形成、运动性和弱酸耐受性的影响。我们发现，部分而非全部钠泵的缺失对铜绿假单胞菌的不同表型具有有害影响。
Mrp钠/质子反向转运蛋白的缺失在该生物体在较高pH和钠浓度环境中的生存和功能能力方面显然很重要，而由nuo基因编码的复合体I的缺失，无论环境的pH和钠浓度如何，对该生物体的生长都有一些一致的影响。
