Key Laboratory of Fertility Regulation, Department of Obstetrics and Gynaecology, The University of Hong Kong - Shenzhen Hospital, Shenzhen, China.
Department of Obstetrics and Gynaecology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, Hong Kong.
BJOG. 2020 May;127(6):720-728. doi: 10.1111/1471-0528.16147. Epub 2020 Feb 25.
To establish the age-specific centiles of serum anti-müllerian hormone (AMH) levels in Chinese women, and to explore the use of multiples of median (MoM) AMH levels for the diagnosis of polycystic ovary syndrome (PCOS).
An observational study.
University-affiliated hospitals and community clinics.
We included 3137 healthy women aged 20-44 years recruited prospectively or who had archived serum samples from previous research projects. Another validation cohort of 751 women with PCOS as well as ovulatory controls, which was a convenient sample of women attending for infertility or menstrual disorders, was also studied.
The serum samples were assayed for AMH by the automated Access AMH assay.
Age-specific reference ranges were constructed on the primary cohort with the Lambda-Mu-Sigma method. The MoM AMH of each subject in the validation cohort was calculated.
Centile curves of serum AMH level against age were established. MoM AMH was significantly higher in women with PCOS than in controls (P < 0.05). The area under the ROC curve was 0.852 (95% confidence interval [CI] 0.825-0.877) (P < 0.0001) for discriminating women with PCOS from ovulatory controls by MoM AMH.
We established a set of year-by-year age-specific reference ranges of serum AMH levels in Chinese women. The MoM AMH derived from this set of reference ranges is a promising tool to replace antral follicle count in the diagnosis of PCOS.
A set of age-specific reference ranges of AMH levels was established in Chinese women. Multiples of median AMH may be used to diagnose PCOS.
建立中国女性血清抗缪勒管激素（AMH）水平的年龄特异性百分位数，并探讨使用 AMH 中位数倍数（MoM）水平进行多囊卵巢综合征（PCOS）的诊断。
观察性研究。
大学附属医院和社区诊所。
我们纳入了 3137 名年龄在 20-44 岁的健康女性，这些女性是前瞻性招募的，或者是之前研究项目的存档血清样本。还研究了另一批 751 名患有 PCOS 和排卵正常的女性作为验证队列，这是一个方便因不孕或月经失调就诊的女性样本。
使用自动化 Access AMH 检测法检测血清 AMH。
采用 Lambda-Mu-Sigma 方法在主要队列中构建年龄特异性参考范围。验证队列中每位受试者的 AMH MoM 均进行计算。
建立了血清 AMH 水平与年龄的百分位曲线。与对照组相比，PCOS 患者的 AMH MoM 明显升高（P<0.05）。MoM AMH 区分 PCOS 患者与排卵正常对照的受试者工作特征曲线下面积为 0.852（95%置信区间 [CI] 0.825-0.877）（P<0.0001）。
我们建立了中国女性血清 AMH 水平的逐年年龄特异性参考范围。本研究参考范围中的 MoM AMH 可能是替代窦卵泡计数用于 PCOS 诊断的有前途的工具。
建立了中国女性 AMH 水平的年龄特异性参考范围。AMH 的中位数倍数可用于诊断 PCOS。
