Instituto Idéia Fértil de Saúde Reprodutiva, Santo André, SP, Brazil.
JBRA Assist Reprod. 2020 May 1;24(2):173-174. doi: 10.5935/1518-0557.20190091.
To compare laboratory results of embryo development from late matured oocytes in relation to mature oocytes in D+0.
We carried out a cross-sectional study during the period from January to December 2018, in which we collected data through medical records analysis. 913 oocytes were collected and divided into 3 groups: group 1 - 643 MII oocytes; group 2 - 119 MI oocytes and; group 3 - 151 PI oocytes. These studied oocytes were from different maternal ages and infertility factors. The analyzed variables were fertilization rate, embryo cleavage, top quality embryos on the third day of development, blastocyst stage, top quality blastocysts, euploid blastocysts, top quality blastocysts and gestation. We documented the data, and performed the statistical analysis using the chi-square test (p<0.05).
All MII oocytes were injected (643); 103/119 MI oocytes and 88/151 PI oocytes that matured late in D + 1, were also injected. The fertilization rate of the three groups did not present statistical difference. The oocytes of group 1 had a statistically proven better prognosis than oocytes from groups 2 and 3 when compared, respectively, embryo cleavage (p=0.000), top quality embryos on the third day of development (p=0.000) and blastocyst formation rate (p=0.004). In the LMO group, there were no euploid embryos and, therefore, there no embryo transfer.
Although late matured oocytes have made blastocyst formation possible, even if in low rates, there were no viable embryos for transfer.
比较晚期成熟卵母细胞与 D+0 期成熟卵母细胞在胚胎发育方面的实验室结果。
我们进行了一项横断面研究，时间为 2018 年 1 月至 12 月，通过病历分析收集数据。共收集了 913 个卵母细胞，并分为 3 组：第 1 组 - 643 个 MII 卵母细胞；第 2 组 - 119 个 MI 卵母细胞；第 3 组 - 151 个 PI 卵母细胞。这些研究卵母细胞来自不同的母体年龄和不孕因素。分析的变量包括受精率、胚胎分裂、第 3 天发育的优质胚胎、囊胚期、优质囊胚、整倍体囊胚、优质囊胚和妊娠。我们记录了数据，并使用卡方检验（p<0.05）进行了统计分析。
所有 MII 卵母细胞均进行了注射（643 个）；119 个 MI 卵母细胞中的 103 个和 151 个 PI 卵母细胞中的 88 个在 D+1 日晚期成熟后也进行了注射。三组的受精率没有统计学差异。与第 2 组和第 3 组相比，第 1 组的卵母细胞具有更好的胚胎分裂（p=0.000）、第 3 天发育的优质胚胎（p=0.000）和囊胚形成率（p=0.004）的统计学预测结果。在 LMO 组中，没有整倍体胚胎，因此没有胚胎移植。
尽管晚期成熟的卵母细胞可以形成囊胚，但即使形成率较低，也没有可用于移植的胚胎。
