Comparing cytotoxicity of propoxur and (Lamiales: Lamiaceae) essential oil against invertebrate (Sf9) and vertebrate (L929) cell lines.

BACKGROUND AND AIM

Attempts to use the plant products are to be an appropriate option due to substantial concerns about human health and environmental problems of using synthetic pesticides. Therefore, the cytotoxicity of essential oil was compared with propoxur against invertebrate (Sf9) and vertebrate (L929) cell lines.

MATERIALS AND METHODS

The cell lines of Sf9 and L929 which were derived from the ovary glands of fall armyworm, (Lepidoptera: Noctuidae) and mouse fibroblast cells, respectively, were obtained from the National Cell Bank of Pasteur Institute of Iran. About a number of 2 × 10 cells were placed into the wells of 96-well plate experiments. Then, appropriate concentrations of essential oil of plant and propoxur added to the wells. The cells were allowed to grow for 3-5 days and estimated the numbers of cells. The cells of control experiment wells contained only cells with dimethyl sulfoxide. All control and treatment experiments repeated at least four replicates.

RESULTS

Propoxur had negative effects on the viability of both invertebrate (Sf9) and vertebrate (L929) cell lines. The cytotoxicity of propoxur against invertebrate (Sf9) and vertebrate (L929) cell lines was gradually increased in accordance with propoxur concentrations. The cytotoxicity of essential oil against vertebrate (L929) cell line was gradually decreased in accordance with plant concentrations, while the cytotoxicity of essential oil against invertebrate (Sf9) cell line was strongly increased in accordance with plant concentrations.

CONCLUSION

Plant essential oil not only had no negative effects but also had boosting effects on vertebrate cell viability. Essential oil of plant had negative effects on invertebrate cell viability with the differences that the products derived from plants possessing of biodegradable and environmentally friendly derivatives, hydrolyzing rapidly in nature, and nearly having no destructive effects on environment, humans, or the mammals.