Bair Kirsten L, Campagnari Anthony A
Department of Microbiology and Immunology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, NY, United States.
The Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, State University of New York, Buffalo, NY, United States.
Front Microbiol. 2020 Jan 15;10:3006. doi: 10.3389/fmicb.2019.03006. eCollection 2019.
Otitis media (OM) is a prevalent pediatric infection characterized by painful inflammation of the middle ear. The Gram-negative diplococcus is a commensal of the nasopharynx and one of three leading causative agents of OM. The most recent work on this multifaceted disease indicates that biofilms and polymicrobial infections play a pivotal role in recurrent and chronic OM, which are difficult to eradicate using standard antibiotic protocols. Although there have been significant advances in OM research, the actual bacterial and viral interactions leading to pathogenesis remain largely uncharacterized. However, colonization and persistence in the nasopharynx is clearly an essential first step. In this study, we assessed the role plays in the co-colonization and persistence of the other major otopathogens, and non-typeable (NTHi). We characterized both monomicrobial and polymicrobial biofilms using an nasopharyngeal colonization model. Biofilm assays were designed to mimic the nasopharynx and bacterial persistence was quantified over time. NTHi showed a steady and significant decline in viability over 20-48 h when this organism was in a dual species biofilm with . However, when was present in the polymicrobial biofilm NTHi survived for 48 h at 10 CFU per mL. In addition, an isogenic catalase-deficient mutant was also fully capable of protecting NTHi from the bactericidal activity of in a polymicrobial biofilm. Our results show that promotes a favorable environment for stable polymicrobial biofilms by enhancing the survival of NTHi in the presence of . These data suggest that colonization with promotes stable co-colonization with other otopathogens.
中耳炎（OM）是一种常见的儿科感染性疾病，其特征为中耳疼痛性炎症。革兰氏阴性双球菌是鼻咽部的共生菌，也是导致中耳炎的三大主要病原体之一。关于这种多方面疾病的最新研究表明，生物膜和混合微生物感染在复发性和慢性中耳炎中起关键作用，而使用标准抗生素方案难以根除这些感染。尽管中耳炎研究取得了重大进展，但导致发病机制的实际细菌和病毒相互作用在很大程度上仍未明确。然而，在鼻咽部的定植和持续存在显然是必不可少的第一步。在本研究中，我们评估了[具体细菌名称]在其他主要耳病原体[具体细菌名称]和不可分型流感嗜血杆菌（NTHi）的共同定植和持续存在中所起的作用。我们使用[具体鼻咽部定植模型名称]对单微生物和多微生物生物膜进行了表征。生物膜试验旨在模拟鼻咽部，并随时间对细菌的持续存在进行定量。当该生物体与[具体细菌名称]处于双物种生物膜中时，NTHi在20 - 48小时内活力呈稳定且显著下降。然而，当[具体细菌名称]存在于多微生物生物膜中时，NTHi在每毫升10 CFU的情况下存活了48小时。此外，一个同基因的[具体细菌名称]过氧化氢酶缺陷突变体在多微生物生物膜中也完全能够保护NTHi免受[具体细菌名称]的杀菌活性影响。我们的结果表明，[具体细菌名称]通过在[具体细菌名称]存在的情况下提高NTHi的存活率，促进了稳定的多微生物生物膜的形成。这些数据表明，[具体细菌名称]的定植促进了与其他耳病原体的稳定共同定植。
