Yun Changjun, Qian Wenjie, Wu Junyi, Yuan Chenxi, Jiang Songzhou, Lv Jinpeng
Department of Orthopedics, Wujin Hospital Affiliated with Jiangsu University, Changzhou, Jiangsu 213000, P.R. China.
The Wujin Clinical College of Xuzhou Medical University, Changzhou, Jiangsu 213000, P.R. China.
Exp Ther Med. 2020 Feb;19(2):923-930. doi: 10.3892/etm.2019.8286. Epub 2019 Dec 5.
Osteoporosis is a severe bone disease characterized by a decrease in the density and structure of bones, with high risks of fractures. Pilose antler peptide (PAP), extracted and purified from deer antlers, can promote regeneration and fracture healing, and strengthen sinews and bone. To determine whether PAP can promote osteoblast development and to elucidate the molecular mechanisms underlying its functions, the present study investigated the effects of PAP on osteoblast proliferation, differentiation and mineralization, and the role of the insulin signaling pathway using MTT assay, alkaline phosphatase activity assay, western blot analysis and reverse transcription-quantitative PCR. The present results suggested that PAP promoted osteoblast proliferation, differentiation and mineralization via the insulin signaling pathway. The effect of PAP on insulin signaling in osteoblasts may be mediated via the ERK pathway and partially by the PI3K/Akt pathway. The present results indicated that PAP could potentially be developed as an alternative treatment strategy for bone diseases related to diabetes characterized by insulin signaling impairment.
骨质疏松症是一种严重的骨骼疾病，其特征是骨密度和结构降低，骨折风险高。从鹿茸中提取和纯化的鹿茸多肽（PAP）可以促进再生和骨折愈合，并强健筋骨。为了确定PAP是否能促进成骨细胞发育并阐明其功能的分子机制，本研究使用MTT法、碱性磷酸酶活性测定、蛋白质印迹分析和逆转录定量PCR研究了PAP对成骨细胞增殖、分化和矿化的影响，以及胰岛素信号通路的作用。目前的结果表明，PAP通过胰岛素信号通路促进成骨细胞增殖、分化和矿化。PAP对成骨细胞中胰岛素信号的影响可能通过ERK途径介导，部分通过PI3K/Akt途径介导。目前的结果表明，PAP有可能被开发为一种替代治疗策略，用于治疗与胰岛素信号受损相关的糖尿病性骨疾病。
