Cheng Renyi, Xie Tao, Ma Wen, Deng Peishen, Liu Chaofeng, Hong Yuchen, Liu Changyu, Tian Jinjun, Xu Yanhua
Department of Orthodontics, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan, China.
Yunnan Key Laboratory of Stomatology, Kunming, China.
Front Bioeng Biotechnol. 2024 Sep 19;12:1460623. doi: 10.3389/fbioe.2024.1460623. eCollection 2024.
Articular cartilage defects often involve damage to both the cartilage and subchondral bone, requiring a scaffold that can meet the unique needs of each tissue type and establish an effective barrier between the bone and cartilage. In this study, we used 3D printing technology to fabricate a tri-phasic scaffold composed of PLA/PCL-PLGA/Mg(OH)₂, which includes a cartilage layer, an osteochondral interface, and a bone layer. The scaffold was filled with Velvet antler polypeptides (VAP), and its characterization was assessed using compression testing, XRD, FTIR, SEM, fluorescence microscopy, and EDS. investigation demonstrated that the scaffold not only supported osteogenesis but also promoted chondrogenic differentiation of fibrocartilage stem cells (FCSCs). n vivo experiments showed that the tri-phasic PLA/PCL-PLGA/Mg(OH)-VAP scaffold together with FCSC, when transplanted to animal models, increased the recovery of osteochondral defects. Those results demonstrate the promising future of illustrated tri-phasic PLA/PCL-PLGA/Mg(OH)-VAP scaffold loaded with FCSCs as a new bone and cartilage tissue engineering approach for osteochondral defects treatment.
关节软骨缺损通常涉及软骨和软骨下骨的损伤，需要一种能够满足每种组织类型独特需求并在骨与软骨之间建立有效屏障的支架。在本研究中，我们使用3D打印技术制造了一种由PLA/PCL-PLGA/Mg(OH)₂组成的三相支架，其包括软骨层、骨软骨界面和骨层。该支架填充有鹿茸多肽（VAP），并使用压缩测试、XRD、FTIR、SEM、荧光显微镜和EDS对其特性进行了评估。研究表明，该支架不仅支持成骨，还促进了纤维软骨干细胞（FCSCs）的软骨分化。体内实验表明，三相PLA/PCL-PLGA/Mg(OH)-VAP支架与FCSC一起移植到动物模型中时，可提高骨软骨缺损的恢复率。这些结果表明，负载FCSCs的图示三相PLA/PCL-PLGA/Mg(OH)-VAP支架作为一种治疗骨软骨缺损的新型骨和软骨组织工程方法具有广阔的前景。
