Feng Juan, Wang Tingfeng
Department of Gynecology and Obstetrics, Weifang Maternity and Child Care Hospital, Weifang, Shandong 261042, P.R. China.
Exp Ther Med. 2020 Feb;19(2):1243-1250. doi: 10.3892/etm.2019.8348. Epub 2019 Dec 18.
Cervical cancer is a common gynecological malignancy with high morbidity worldwide. MicroRNAs (miRNAs) serve critical roles in cervical cancer progression. Accumulating evidence indicates that miR-873 functions as a tumor suppressor in certain types of cancer. However, the function and mechanism of miR-873 in the progression of cervical cancer have not been completely elucidated. In the present study, the role and mechanism of miR-873 in the proliferation, migration and invasion of cervical cancer cells were investigated. miR-873 expression was markedly decreased in cervical cancer, while glioma-associated oncogene homolog 1 (GLI1) was found to be a direct target of miR-873 by conducting dual-luciferase reporter assays. Furthermore, miR-873 overexpression reduced the expression of GLI1, and decreased the proliferation, metastasis and epithelial-mesenchymal transition of cancer cells. In rescue experiments, overexpression of GLI1 in cervical cancer cells effectively reversed the inhibitory effect induced by miR-873 mimics. Therefore, the results of the present study suggested that miR-873 functions as a tumor suppressor miRNA, and future studies should address its potential application in the treatment of cervical cancer.
宫颈癌是一种常见的妇科恶性肿瘤，在全球范围内发病率很高。微小RNA（miRNA）在宫颈癌进展中发挥关键作用。越来越多的证据表明，miR-873在某些类型的癌症中起肿瘤抑制作用。然而，miR-873在宫颈癌进展中的功能和机制尚未完全阐明。在本研究中，研究了miR-873在宫颈癌细胞增殖、迁移和侵袭中的作用及机制。miR-873在宫颈癌中表达明显降低，通过双荧光素酶报告基因实验发现胶质瘤相关癌基因同源物1（GLI1）是miR-873的直接靶点。此外，miR-873过表达降低了GLI1的表达，并减少了癌细胞的增殖、转移和上皮-间质转化。在拯救实验中，宫颈癌细胞中GLI1的过表达有效逆转了miR-873模拟物诱导的抑制作用。因此，本研究结果表明miR-873作为一种肿瘤抑制性miRNA发挥作用，未来的研究应探讨其在宫颈癌治疗中的潜在应用。
