印度尼西亚爪哇族代谢综合征患者脂蛋白脂肪酶基因变异的年龄分层

Age Stratification in Genetic Variation of Lipoprotein Lipase in Metabolic Syndrome Javanese Ethnics of Indonesia.

作者信息

Mus Rosdiana, Sadewa Ahmad Hamim, Hastuti Pramudji, Puspasari Anggelia, Maharani Citra, Setyawati Ika

机构信息

Technology of Laboratorium Medis, Faculty of Pharmacy, Hospital Technology and Informatics, Universitas Mega Rezky, Makassar, Indonesia.

Department of Biochemistry, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.

出版信息

Open Access Maced J Med Sci. 2019 Oct 13;7(21):3540-3545. doi: 10.3889/oamjms.2019.844. eCollection 2019 Nov 15.

DOI:10.3889/oamjms.2019.844

PMID:32010373

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6986532/

Abstract

BACKGROUND

Metabolic syndrome (Met-S) that caused by heredity and Lipoprotein Lipase (LPL). LPL is involved in the metabolism of serum lipids. Variations in LPL alter enzyme activity, and the most common variations are LPL +495 T > G and LPL Pvu II C > T.

AIM

This study aimed to identify the role of LPL +495 T > G and LPL PvuII C > T gene variations in subjects with Met-S in Javanese ethnic based on age stratification.

METHODS

We recruited 160 participants of Javanese ethnicity consisting of 80 cases and 80 control subjects. Met-S was diagnosed according to the criteria of NCEP ATP III. Peripheral blood samples were collected to determine biochemical parameters. Screening for both polymorphisms was made by PCR-RFLP.

RESULTS

Results found that genotype and allele frequencies for LPL +495 T > G were not significantly different between Met-S and controls with and without age stratification. In LPL PvuII C > T based on age stratification, there were significant differences between TT vs CC, recessive and dominant models in Met-S and control. In the age group > 45 years CC genotypes and TC+CC had increased risk of Met-S compared to TT genotypes. In summary, there was no significant association between LPL +495 T > G gene variation with Met-S.

CONCLUSION

In LPL PvuII gene variation, TC + CC is the risk genotype of Met-S in the age group > 45 years.

摘要

背景

代谢综合征（Met-S）由遗传因素和脂蛋白脂肪酶（LPL）引起。LPL参与血清脂质的代谢。LPL的变异会改变酶的活性，最常见的变异是LPL +495 T>G和LPL Pvu II C>T。

目的

本研究旨在基于年龄分层确定LPL +495 T>G和LPL PvuII C>T基因变异在爪哇族代谢综合征患者中的作用。

方法

我们招募了160名爪哇族参与者，其中包括80例病例和80名对照受试者。根据NCEP ATP III标准诊断代谢综合征。采集外周血样本以确定生化参数。通过PCR-RFLP对两种多态性进行筛查。

结果

结果发现，无论是否进行年龄分层，代谢综合征患者与对照之间LPL +495 T>G的基因型和等位基因频率均无显著差异。基于年龄分层的LPL PvuII C>T中，代谢综合征患者与对照在TT与CC、隐性和显性模型之间存在显著差异。在年龄>45岁的组中，与TT基因型相比，CC基因型以及TC+CC基因型的代谢综合征风险增加。总之，LPL +495 T>G基因变异与代谢综合征之间无显著关联。