Olatinwo Rabiu, So Chi-Leung, Eberhardt Thomas L
USDA Forest Service, Southern Research Station, Pineville, LA, USA.
School of Renewable Natural Resources, LSU AgCenter, Baton Rouge, LA, USA.
Mycobiology. 2019 Nov 21;47(4):506-511. doi: 10.1080/12298093.2019.1686944. eCollection 2019.
We investigated the antifungal activities of an endophytic fungus identified as , found on a loblolly ( L.) pine bolt in Louisiana during routine laboratory microbial isolations. The specific objectives were to determine the inhibitory properties of secondary metabolites (crude extract) on the mycelial growth of a brown-rot fungus and a white-rot fungus , and to determine the effective concentration of crude preparation against the two decay fungi . Results show the crude preparation of from liquid culture possesses significant mycelial growth inhibitory properties that are concentration dependent against the brown-rot and white-rot fungi evaluated. An increase in the concentration of secondary metabolites significantly decreased the mycelial growth of both wood decay fungi. was more sensitive to the inhibitory effect of the secondary metabolites than . Identification of specific secondary metabolites, and analysis of their efficacy/specificity warrants further study. Findings from this work may provide the first indication of useful roles for species in a forest environment, and perhaps a future potential in the development of biocontrol-based wood preservation systems.
我们对一种在路易斯安那州火炬松（Pinus taeda L.）原木上发现的内生真菌进行了抗真菌活性研究，该真菌是在常规实验室微生物分离过程中被鉴定出来的。具体目标是确定该内生真菌次生代谢产物（粗提物）对褐腐真菌和白腐真菌菌丝生长的抑制特性，并确定该粗提物对这两种腐朽真菌的有效浓度。结果表明，从液体培养物中获得的该内生真菌粗提物具有显著的菌丝生长抑制特性，且对所评估的褐腐真菌和白腐真菌的抑制作用呈浓度依赖性。次生代谢产物浓度的增加显著降低了两种木材腐朽真菌的菌丝生长。相较于[白腐真菌名称]，[褐腐真菌名称]对次生代谢产物的抑制作用更为敏感。鉴定特定的次生代谢产物及其功效/特异性分析值得进一步研究。这项工作的发现可能首次表明该内生真菌物种在森林环境中的有益作用，以及在基于生物防治的木材防腐系统开发方面的潜在未来价值。
