Zikeli Florian, Vettraino Anna Maria, Biscontri Margherita, Bergamasco Sara, Palocci Cleofe, Humar Miha, Romagnoli Manuela
Department for Innovation in Biological, Agro-Food and Forest Systems (DIBAF), University of Tuscia, 01100 Viterbo, Italy.
Department of Chemistry, Sapienza University of Rome, Piazzale A. Moro 5, 00185 Rome, Italy.
Polymers (Basel). 2023 Jun 17;15(12):2713. doi: 10.3390/polym15122713.
After decades of utilization of fossil-based and environmentally hazardous compounds for wood preservation against fungal attack, there is a strong need to substitute those compounds with bio-based bioactive solutions, such as essential oils. In this work, lignin nanoparticles containing four essential oils from thyme species (, , , and Demeter) were applied as biocides in in vitro experiments to test their anti-fungal effect against two white-rot fungi ( and ) and two brown-rot fungi ( and ). Entrapment of essential oils provided a delayed release over a time frame of 7 days from the lignin carrier matrix and resulted in lower minimum inhibitory concentrations of the essential oils against the brown-rot fungi (0.30-0.60 mg/mL), while for the white-rot fungi, identical concentrations were determined compared with free essential oils (0.05-0.30 mg/mL). Fourier Transform infrared (FTIR) spectroscopy was used to assess the fungal cell wall changes in the presence of essential oils in the growth medium. The results regarding brown-rot fungi present a promising approach for a more effective and sustainable utilization of essential oils against this class of wood-rot fungi. In the case of white-rot fungi, lignin nanoparticles, as essential oils delivery vehicles, still need optimization in their efficacy.
在使用基于化石的、对环境有害的化合物来保护木材免受真菌侵害数十年之后，迫切需要用生物基生物活性溶液（如精油）来替代这些化合物。在这项工作中，含有四种百里香属植物精油（、、和得墨忒耳）的木质素纳米颗粒在体外实验中用作杀菌剂，以测试它们对两种白腐真菌（和）和两种褐腐真菌（和）的抗真菌效果。精油包封在7天的时间范围内从木质素载体基质中实现了缓释，并且导致精油对褐腐真菌的最低抑菌浓度降低（0.30 - 0.60毫克/毫升），而对于白腐真菌，与游离精油相比确定了相同的浓度（0.05 - 0.30毫克/毫升）。傅里叶变换红外（FTIR）光谱用于评估生长培养基中存在精油时真菌细胞壁的变化。关于褐腐真菌的结果为更有效和可持续地利用精油对抗这类木材腐朽真菌提供了一种有前景的方法。在白腐真菌的情况下，木质素纳米颗粒作为精油递送载体，其功效仍需要优化。
