Ou Tongwen, Zhou Zejun, Turner David P, Zhu Baoli, Lilly Michael, Jiang Wei
Department of Urology, Capital Medical University Affiliated XuanWu Hospital, Beijing, China.
State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, China.
Front Oncol. 2020 Jan 15;9:1532. doi: 10.3389/fonc.2019.01532. eCollection 2019.
The environmental factors for promoting prostate cancer (PCa) recurrence remain unknown. A retrospective cross-sectional study was conducted in healthy men ( = 12) and PCa patients undergoing prostatectomy ( = 27). Plasma preoperative level of total cell-free bacterial 16S rDNA, a marker of microbial translocation, was evaluated by qPCR. Plasma levels of prostate-specific antigen (PSA) were evaluated by ELISA. Similar degrees of microbial translocation were found in healthy men and patients. However, the levels of microbial 16S rDNA were increased in patients with cancer relapse ( = 10) compared to patients without relapse ( = 17) after prostatectomy. Furthermore, the levels of microbial 16S rDNA were marginally increased in patients with pT3 or pT4 tumors compared to those with pT 2 or less. The levels of microbial 16S rDNA tended to increase in patients with higher pathologic tumor stage, Gleason score, and margin and lymph node involvements; but these differences did not reach significance. The plasma 16S rDNA levels increased in patients with PCa who have biochemical recurrence and 16S rDNA levels were higher in patients with higher-grade PCa.
促进前列腺癌（PCa）复发的环境因素尚不清楚。对健康男性（n = 12）和接受前列腺切除术的PCa患者（n = 27）进行了一项回顾性横断面研究。通过qPCR评估术前血浆中总游离细菌16S rDNA水平，这是微生物易位的一个标志物。通过ELISA评估前列腺特异性抗原（PSA）的血浆水平。在健康男性和患者中发现了相似程度的微生物易位。然而，与前列腺切除术后未复发的患者（n = 17）相比，癌症复发患者（n = 10）的微生物16S rDNA水平升高。此外，与pT2或更低分期的患者相比，pT3或pT4肿瘤患者的微生物16S rDNA水平略有升高。微生物16S rDNA水平在病理肿瘤分期、Gleason评分、切缘和淋巴结受累程度较高的患者中呈上升趋势；但这些差异未达到显著水平。发生生化复发的PCa患者血浆16S rDNA水平升高，高级别PCa患者的16S rDNA水平更高。
