Department of Neurosurgery, Jining No.1 People's Hospital, Jining, China.
Department of Occupational Medicine, Weifang People's Hospital, Weifang, China.
Phytother Res. 2020 Mar;34(3):591-600. doi: 10.1002/ptr.6545. Epub 2020 Feb 3.
Icariin (ICA) is obtained from Epimedium brevicornu maxim and exploited to remedy miscellaneous cancers. But the role of ICA in medulloblastoma remains hazy. The research delved into the antitumor activity of ICA in medulloblastoma DAOY cells. ICA with diverse concentrations was utilized to stimulate DAOY cells, and the biological functions of ICA in medulloblastoma DAOY cells were examined. Then, the relative SPARC expression was determined in ICA-managed DAOY cells, and the pc-SPARC vector was transfected into DAOY cells to further probe the influence of SPARC and JAK1/STAT3 and PI3K/AKT pathways in ICA-managed DAOY cells. A xenograft model was established to investigate the function of ICA in vivo. ICA restrained cell viability, expedited apoptosis, prohibited cell migration and invasion, and meanwhile affected the associative factors expression in DAOY cells. Additionally, SPARC expression was declined in ICA-stimulated DAOY cells. Overexpressed SPARC reversed the functions of ICA in above-involved cell behaviors of DAYO cells and the correlative protein levels. Besides, ICA notably frustrated JAK1/STAT3 and PI3K/AKT activations in DAOY cells. Beyond that, ICA prohibited tumor formation in vivo. The results concluded that ICA exhibited the antitumor activity in DAOY cells via decreasing SPARC and inactivating JAK1/STAT3 and PI3K/AKT pathways.
朝藿定（ICA）是从淫羊藿中提取的，用于治疗各种癌症。但是 ICA 在髓母细胞瘤中的作用还不清楚。本研究探讨了 ICA 对髓母细胞瘤 DAOY 细胞的抗肿瘤活性。用不同浓度的 ICA 刺激 DAOY 细胞，检测 ICA 对髓母细胞瘤 DAOY 细胞的生物学功能。然后，测定 ICA 处理的 DAOY 细胞中相对 SPARC 的表达，并用 pc-SPARC 载体转染 DAOY 细胞，进一步探讨 SPARC 以及 JAK1/STAT3 和 PI3K/AKT 通路在 ICA 处理的 DAOY 细胞中的影响。建立异种移植模型，研究 ICA 在体内的功能。ICA 抑制细胞活力，促进细胞凋亡，抑制细胞迁移和侵袭，同时影响 DAOY 细胞相关因子的表达。此外，ICA 刺激的 DAOY 细胞中 SPARC 的表达下降。过表达 SPARC 逆转了 ICA 对 DAYO 细胞上述细胞行为及相关蛋白水平的作用。此外，ICA 显著抑制了 DAOY 细胞中 JAK1/STAT3 和 PI3K/AKT 的激活。此外，ICA 抑制了体内肿瘤的形成。结果表明，ICA 通过降低 SPARC 并激活 JAK1/STAT3 和 PI3K/AKT 通路，在 DAOY 细胞中表现出抗肿瘤活性。
