Nayak Prathvi P, Biranthabail Dhanashree, Shenoy Shalini, Kotian Shashidhar M
Manipal Academy of Higher Education, Manipal, India.
J Infect Dev Ctries. 2018 Nov 30;12(11):985-990. doi: 10.3855/jidc.9966.
Drug-resistant Enterococcus species is a persisting clinical problem and may serve as a reservoir of resistant genes. The present study was undertaken in Mangalore, India to know the antibiogram and genetic relatedness of Enterococcus spp. isolated from clinical samples.
A total of 150 non-repetitive Enterococcus spp. isolated from clinical samples were subjected to antimicrobial susceptibility testing by Kirby Bauer disk diffusion method. Molecular typing of the isolates was done by Random amplification of polymorphic DNA (RAPD).
Among the 150 isolates, 79 were from urine, 68 from pus and three from blood samples. Of this 58.7 % were E. faecalis and the remaining were E. faecium. Urinary isolates of E. faecium showed a higher percentage of antibiotic resistance when compared to E. faecium isolates from pus (p < 0.001). E. faecium from blood samples were resistant to ampicillin, penicillin, ciprofloxacin and were sensitive to vancomycin and teicoplanin. E. faecalis blood isolates were resistant to ciprofloxacin, penicillin, and erythromycin. 73% of Enterococcus isolates from pus were resistant to erythromycin. All the Enterococcus spp. were sensitive to vancomycin. Among the total Enterococcus isolates 44 were high-level aminoglycoside resistant (HLAR) by disc diffusion method which corresponded to MIC of > 500 µg/mL for gentamicin and > 1000 µg/mL for streptomycin. These isolates were subjected to RAPD, which showed similarity and differences in the banding patterns.
Our study showed a baseline resistance among Enterococcus spp. in our area, which poses a challenge to the treating physicians and a reservoir for transmission of antibiotic resistant genes.
耐药肠球菌属是一个持续存在的临床问题，可能成为耐药基因的储存库。本研究在印度芒格洛尔开展，旨在了解从临床样本中分离出的肠球菌属的抗菌谱和基因相关性。
采用 Kirby Bauer 纸片扩散法，对从临床样本中分离出的 150 株非重复肠球菌属菌株进行药敏试验。通过随机扩增多态性 DNA（RAPD）对分离株进行分子分型。
在 150 株分离株中，79 株来自尿液，68 株来自脓液，3 株来自血液样本。其中，58.7%为粪肠球菌，其余为屎肠球菌。与来自脓液的屎肠球菌分离株相比，尿液中的屎肠球菌分离株显示出更高比例的抗生素耐药性（p < 0.001）。血液样本中的屎肠球菌对氨苄西林、青霉素、环丙沙星耐药，对万古霉素和替考拉宁敏感。粪肠球菌血液分离株对环丙沙星、青霉素和红霉素耐药。73%的来自脓液的肠球菌分离株对红霉素耐药。所有肠球菌属对万古霉素敏感。通过纸片扩散法，在所有肠球菌分离株中，有 44 株对高水平氨基糖苷类耐药（HLAR），对应庆大霉素 MIC > 500 µg/mL，链霉素 MIC > 1000 µg/mL。对这些分离株进行 RAPD 分析，结果显示条带模式存在相似性和差异。
我们的研究显示了我们地区肠球菌属的基线耐药情况，这给治疗医生带来了挑战，也是抗生素耐药基因传播的储存库。
