AtallahBenson Leila, Merly Liza, Cray Carolyn, Hammerschlag Neil
Department of Marine Ecosystems and Society, Rosenstiel School of Atmospheric Science, University of Miami, 4600 Rickenbacker Causeway, Miami, Florida, 33149, USA.
Shark Research and Conservation Program, University of Miami, 4600 Rickenbacker Causeway, Miami, Florida, 33149, USA.
J Aquat Anim Health. 2020 Jun;32(2):77-82. doi: 10.1002/aah.10100. Epub 2020 Mar 11.
Serum protein electrophoresis (EPH) is used to assess relative concentrations of blood proteins in clinical and biological studies. Serum EPH fractions have been determined for elasmobranchs using mammalian albumin, alpha 1-, alpha 2-, beta-, and gamma-globulin fractions, and have been deemed fractions 1 through 5, respectively. However, serum EPH fraction concentration reference intervals (RIs) have not been widely established for different elasmobranch species. In this study, RIs for fractions 1 through 5 were determined from 45 wild-caught Nurse Sharks Ginglymostoma cirratum (27 females and 23 males) in South Florida. Serum samples were isolated from whole blood following caudal venipuncture. Body condition was also measured in the field to assess the relative health of the individuals sampled. There was no relationship between body condition and serum EPH fraction concentrations. In addition, there was no difference in body condition or serum EPH fraction concentrations between females and males. Total solids and total protein values were significantly different (P < 0.001). Nurse Shark serum EPH fraction 1 was found within the mammalian albumin migrating band distance and was negligible. Fraction 2 showed no peak in the mammalian alpha 1-globulin range. A thin, medium peak in the mammalian alpha 2-globulin range represented fraction 3. In the mammalian beta-globulin range, fraction 4 consisted of the majority of protein observed. It was represented by a smooth, broad peak. A short, medium broad peak in the mammalian gamma-globulin range represented fraction 5. The Nurse Shark serum EPH fraction RIs provided in this study may be utilized to clinically evaluate the health of Nurse Sharks in captivity and in the wild, and to compare the health of their populations around the world experiencing various anthropogenic stressors and other environmental impacts.
血清蛋白电泳（EPH）用于临床和生物学研究中评估血液蛋白的相对浓度。使用哺乳动物的白蛋白、α1-、α2-、β-和γ-球蛋白组分来测定板鳃亚纲动物的血清EPH组分，分别将其视为组分1至5。然而，不同板鳃亚纲物种的血清EPH组分浓度参考区间（RIs）尚未广泛确立。在本研究中，从南佛罗里达州45条野生捕获的护士鲨（Ginglymostoma cirratum）（27只雌性和23只雄性）中确定了组分1至5的RIs。通过尾静脉穿刺从全血中分离出血清样本。还在现场测量了身体状况，以评估所采样个体的相对健康状况。身体状况与血清EPH组分浓度之间没有关系。此外，雌性和雄性在身体状况或血清EPH组分浓度方面没有差异。总固体和总蛋白值有显著差异（P < 0.001）。发现护士鲨血清EPH组分1在哺乳动物白蛋白迁移带距离范围内，且可忽略不计。组分2在哺乳动物α1-球蛋白范围内未显示出峰。在哺乳动物α2-球蛋白范围内有一个薄的中等峰代表组分3。在哺乳动物β-球蛋白范围内，组分4由观察到的大部分蛋白质组成。它由一个平滑、宽阔的峰表示。在哺乳动物γ-球蛋白范围内有一个短的中等宽峰代表组分5。本研究中提供的护士鲨血清EPH组分RIs可用于临床评估圈养和野生护士鲨的健康状况，并比较世界各地经历各种人为压力源和其他环境影响的护士鲨种群的健康状况。
